| 白花蛇舌草中对香豆酸提取工艺研究及其含量测定 |
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| 引用本文： | 刘珊珊,金向群,高丹.白花蛇舌草中对香豆酸提取工艺研究及其含量测定[J].特产研究,2006,28(1):21-24. |
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| 作者姓名： | 刘珊珊 金向群 高丹 |
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| 作者单位： | 吉林大学药学院,吉林,长春,130021 |
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| 基金项目： | 吉林省科技厅项目(2000050910) |
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| 摘 要： | 目的以对香豆酸为指标性成分,研究白花蛇舌草的最佳提取纯化工艺。方法采用有机溶酶提取与大孔树脂动态吸附相结合的方法,用高效液相法测定所得的样品中对香豆酸含量,最终确定最佳工艺。结果以90%乙醇提取,提取液回收乙醇后,经大孔树脂柱纯化,收集40%乙醇洗脱液,回收乙醇,浓缩、干燥,干燥提取物再经乙酸乙酯加热回流,此提取物的纯度较为理想。
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| 关 键 词： | 白花蛇舌草 对香豆酸 提取纯化 高效液相色谱法 |
| 文章编号： | 1001-4721（2006）01-0021-04 |
| 收稿时间： | 2005-12-18 |
| 修稿时间： | 2005年12月18 |
Study on Extraction Methods of p- Coumaric Acid in Oldenlandia diffusa (willd.) Roxb and Determine the Content by HPLC |
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| LIU Shan-shan,JIN Xiang-qun,GAO Dan.Study on Extraction Methods of p- Coumaric Acid in Oldenlandia diffusa (willd.) Roxb and Determine the Content by HPLC[J].Special Wild Economic Animal and Plant Research,2006,28(1):21-24. |
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| Authors: | LIU Shan-shan JIN Xiang-qun GAO Dan |
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| Institution: | College of Pharmaey,Jilin University. Changehun 130021, China |
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| Abstract: | Objective To study the best method for extraction of p-coumaric acid in Oldenlandia diffusa(willd.) Roxb.Methods With different menstruum and macroporous adsorption resin to extract p-comaric acid in Oldenlandia diffusa(willd.) Roxb,and the contents were scaned by hplc,to select the best method.Results With 90% ethanol extraction,then to absorb and purify with macroporous resin,collect 40% ethanol elution,heat up the back flow with ethyl acetate.by these sereies of crafts,the content of p-coumaric is ideal. |
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| Keywords: | Oldenlandia diffusa(willd ) Roxb p-comaric acid extraction and purification HPLC |
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