| 甘蔗胚性细胞团诱导影响因子研究 |
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| 引用本文： | 李松,杨荣仲,廖江雄,刘丽敏,余坤兴,淡明,刘红坚,杨柳,谭芳,戴友铭.甘蔗胚性细胞团诱导影响因子研究[J].广西农业科学,2010,41(6):519-522. |
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| 作者姓名： | 李松 杨荣仲 廖江雄 刘丽敏 余坤兴 淡明 刘红坚 杨柳 谭芳 戴友铭 |
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| 作者单位： | 1. 中国农业科学院甘蔗研究中心,广西南宁,530007;广西甘蔗研究所,南宁,530007
2. 广西大学,南宁,530005 |
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| 基金项目： | 广西自然科学基金项目，广西农业科学院科技发展基金项目，广西科技创新能力与条件建设项目，广西甘蔗研究所基本科研业务专项项目 |
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| 摘 要： | 以不同基因型甘蔗品种新台糖22号、新台糖16号、桂糖21号及拔地拉为供试材料,以近生长点0～3 cm的嫩叶鞘组织为外植体、MS固体培养基为基本培养基,研究诱导初期不同分化刺激培养时间和不同激素组合（2,4-D 1.0～7.0 mg/L、ABA 0～2.0 mg/L）对愈伤组织胚性细胞团诱导或分化的影响。结果表明,在MS＋6-BA 1.5 mg/L＋NAA 0.5mg/L培养基中对嫩叶鞘愈伤组织进行分化刺激培养24～72 h,可显著提高胚性细胞团的诱导率,以培养48 h的诱导率最高,平均诱导率为44.4%,比对照增加15.7个百分点;在不同激素组合培养基中,以在培养基MS＋2,4-D 3.0 mg/L＋ABA0.5 mg/L中培养的不同甘蔗品种胚性细胞团诱导率较高、生长较好,其次是MS＋2,4-D 3.0 mg/L;在分化培养基MS＋6-BA 1.5 mg/L＋NAA 0.1 mg/L中对来自不同诱导培养基的胚性细胞团进行分化培养,结果发现在MS＋2,4-D 3.0 mg/L＋ABA 0.5 mg/L和MS＋2,4-D 3.0 mg/L中诱导的胚性细胞团的绿苗分化率较高,不同甘蔗品种平均绿苗分化率分别为65.0%、64.9%,但绿苗生根率较低。
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| 关 键 词： | 甘蔗 胚性细胞团 诱导 激素 分化 绿苗 |
Factors affecting induction of callus and embryogenic cell cluster derived from young leaf sheath of sugarcane |
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| LI Song,YANG Rong-zhong,LIAO Jiang-xiong,LIU LI-min,YU Kun-xing,DAN Ming,LIU Hong-jian,YANG Liu,TAN Fang,DAI You-ming.Factors affecting induction of callus and embryogenic cell cluster derived from young leaf sheath of sugarcane[J].Guangxi Agricultural Sciences,2010,41(6):519-522. |
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| Authors: | LI Song YANG Rong-zhong LIAO Jiang-xiong LIU LI-min YU Kun-xing DAN Ming LIU Hong-jian YANG Liu TAN Fang DAI You-ming |
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| Institution: | LI Song1,2,YANG Rong-zhong1,LIAO Jiang-xiong1,LIU LI-min1,YU Kun-xing1,DAN Ming1,LIU Hong-jian1,YANG Liu3,TAN Fang1,DAI You-ming1,2(1 Sugarcane Research Center,Chinese Academy of Agricultural Sciences,Nanning,Guangxi 530007,China,2 Guangxi Sugarcane Research Institute,Nanning 53007,2 Guangxi University,Nanning 530005,China) |
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| Abstract: | In the present study,different sugarcane varieties,viz.,ROC22,ROC16,GT21 and Badila were used for investigating the effects of different differentiation incentive culture time at early induction stage,and hormone combinations(2,4-D 1.0-7.0 mg/L,ABA 0-2.0 mg/L) on induction or differentiation of embryogenic cell cluster.The 0-3 cm of young leaf sheath(near to growing point) was used as explants,and solid MS was used as the basic culture medium.The induction rate of embyrogenic cell cluster derived from callu... |
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| Keywords: | sugarcane embryogenic cell cluster induction hormone differentiation green plantlets |
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