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| 蓝狐自咬症RAPD标记的筛选 | |
| 引用本文： | 任二军,刘宗岳,张铁涛,邢秀梅,杨福合.蓝狐自咬症RAPD标记的筛选[J].特产研究,2011,33(3):1-5. |
| 作者姓名： | 任二军 刘宗岳 张铁涛 邢秀梅 杨福合 |
| 作者单位： | 1. 中国农业科学院特产研究所吉林省特种经济动物分子生物学重点实验室吉林省特种经济动物分子生物学省部共建实验室,吉林吉林132109;石家庄市农林科学研究院,石家庄050041 2. 中国农业科学院特产研究所吉林省特种经济动物分子生物学重点实验室吉林省特种经济动物分子生物学省部共建实验室,吉林吉林,132109 3. 中国农业科学院饲料研究所,北京,100081 |
| 基金项目： | 国家科技支撑计划项目(2006BAD06B06) |
| 摘 要： | 蓝狐是一种珍贵的毛皮动物，自咬症的发生严重影响了蓝狐的生长发育和毛皮质量，给蓝狐养殖业带来了巨大的经济损失。为了寻找与蓝狐自咬症紧密连锁的遗传标记，采用RAPD技术对健康和自咬蓝狐分子标记进行筛选。从120条随机引物中筛选出在健康蓝狐和自咬蓝狐中扩增出差异条带的6条引物，分别为S77、S79、S88、S472、S474、S485。经个体验证，S472、S4852条随机引物在健康蓝狐和自咬蓝狐出现特异条带的个体数量差异达极显著水平（P〈0．01），可作为蓝狐自咬症的分子标记，为蓝狐自咬症的深入研究奠定了基础。 |
| 关 键 词： | 蓝狐 自咬症 RAPD 分子标记 |
Screening the RAPD Marker Linked to Self-biting Disease of Blue Fox |
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| REN Er-jun,LIU Zong-yue,ZHANG Tie-tao,XING Xiu-mei,YANG Fu-he.Screening the RAPD Marker Linked to Self-biting Disease of Blue Fox[J].Special Wild Economic Animal and Plant Research,2011,33(3):1-5. | |
| Authors: | REN Er-jun LIU Zong-yue ZHANG Tie-tao XING Xiu-mei YANG Fu-he |
| Institution: | REN Er-jun1,2,LIU Zong-yue1,ZHANG Tie-tao3,XING Xiu-mei1,YANG Fu-he11.Institute of Special Economic Animal and Plant Science,The Chinese Academy of Agricultural Sciences,Jinlin Provincial Key Laboratory for Molecular Biology of Special Economic Animals,State Key Laboratory for Molecular Biology of Special Economic Animal,Jilin 132109,China,2.Shijiazhuang Academy of Agricultural and Forestry Science,Shijiazhuang 050041,3.Feed Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,Ch... |
| Abstract: | Blue fox is a precious fur-bearing animal.The self-biting disease of blue foxes can cause serious problem on their growth and the pelt-quality,eventually cause considerable economic losses in blue fox farming industry.In order to identify the genetic marker linked to self-biting disease,RAPD was used to analyze molecular marker of the healthy and self-biting blue foxes.6 primers amplified specific fragments in healthy foxes and self-biting ones were screened from 120 random primers by RAPD,they were S77、S79、S88、S472、S474、S485 respectively.Detected by individuals,the numbers with specific fragments amplified by primer S472 and primer S485 in the healthy foxes and self-biting foxes showed significantly difference(P<0.01),theses two primers could be used as molecular marker of self-biting disease.The results provided theoretical basis for further study on the self-biting disease of blue foxes. |
| Keywords: | Bblue fox Self-biting RAPD Molecular marker |
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