河南大尾寒羊血液蛋白多态性与多羔性的关系研究

采用PAGE对河南大尾寒羊的血红蛋白(Hb)、转铁蛋白(Tf)进行多态性检测,同时以美利奴羊作为对照。结果表明:河南大尾寒羊Hb位点的HbB基因和HbBB基因型频率分别为0.694 5和0.500 0,且两品种差异显著,两群体中HbB基因均占优势。但大尾寒羊的HbB基因频率比美利奴羊群体高26.27%,HbBB基因型频率高66.7%,可能与其多羔性存在相关性。在36份河南大尾寒羊Tf血样中共检出5种基因(TfA,TfB,TfC,TfD,TfE)、10种表现型(TfAB,TfAC,TfAD,TfBB,TfBC,TfBD,TfCC,TfCD,TfCE,TfDD),TfCD产羔数最高为3个,TfCE的产羔数量最低为2个,不同表现型母羊的产羔数存在一定的差异。

Relationship between Blood Protein Polymorphism and Prolificacy of Long-tail Han Sheep in H enan Province

Taking Merion monotonous sheep as CK,the morphologism of Hb and TF of Long-tail Han Sheep in Henan province was tested by non-continuous vertical PAGE.The result showed that HbB gene and HbBB genotype frequence of Long-tail Han Sheep were 0.6945 and 0.5000,respectively,presenting significant difference between the two breeds(P<0.05,P<0.01).The HbB genes in both multi-lamb Long-tail Han Sheep and single-lamb Merino monotocous sheep were in superiority,but the Hb gene frequence of Long-tail Han Sheep was 26.27% higher than that of Merino monotocous sheep(P<0.01);HbBB genotype frequence of Long-tail Han Sheep is 66.7% higher than that of Merino monotocous sheep(P<0.01),which may relate to the multi-lamb feature.The Tf polymorphism test showed that among 36 bloods of Long-tail Han Sheep,five genes(TfA,TfB,TfC,TfD and TfE) and ten phenotypes(TfAB,TfAC,TfAD,TfBB,TfBC,TfBD,TfCC,TfCD,TfCE andTfDD) were detected.The highest lamb-breeding number is three from TfCD,and the lowest lamb-breeding number is two from TfCE.There existed some difference of lamb-breeding number between different phenotypes of female sheep,but not significant.