| 铜绿假单胞菌脂肪酶Lipase基因的原核表达(英文) |
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| 引用本文： | 张煜星,武寒雪,祝建波,刘焕,周鹏.铜绿假单胞菌脂肪酶Lipase基因的原核表达(英文)[J].农业科学与技术,2008,9(5):59-62. |
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| 作者姓名： | 张煜星 武寒雪 祝建波 刘焕 周鹏 |
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| 作者单位： | 中国热带农业科学院热带生物技术研究所国家重点实验室;石河子大学生命科学学院农业生物技术重点实验室;新疆农业科学院微生物应用研究所 |
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| 基金项目： | 国家高技术研究发展计划(863计划)
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兵团博士基金
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| 摘 要： | Objective] The aim of this study was to investigate the prokaryotic expression of pseudomonas aeruginosa Lipase gene.Method]Lipase gene was amplified by PCR from the genome DNA of pseudomonas aeruginosa,and its nucleotide sequence was determined.The prokaryotic expression vector of Lipase gene was constructed by the gene recombination technique.The protein expression was induced for 4 hours by IPTG with the final concentration of 1.0 mmol/L,and then SDS-PAGE electrophoresis was analyzed.Result]The sequence of mature peptides in Lipase gene cloned from pseudomonas aeruginosa had a 99.36% homology with that of pseudomonas aeruginosa lipase submitted in NCBI,so the prokaryotic expression vector of Lipase gene pET32a-Lip was successfully constructed.Furthermore,the results of SDS-PAGE electrophoresis showed that the target gene was expressed highly and effectively.Conclusion]The cloned pseudomonas aeruginosa lipase with its signal peptide could be normally expressed in E.coli and also used for further study.
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| 关 键 词： | Pseudomonas aeruginosa Lipase Prokaryotic expression |
Prokaryotic Expression of Pseudomonas Aeruginosa Lipase Gene |
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| ZHANG Yu-xing,Wu Han-xue,ZHU Jian-bo,LIU Huan,ZHOU Peng.Prokaryotic Expression of Pseudomonas Aeruginosa Lipase Gene[J].Agricultural Science ＆ Technology,2008,9(5):59-62. |
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| Authors: | ZHANG Yu-xing Wu Han-xue ZHU Jian-bo LIU Huan ZHOU Peng |
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| Institution: | 1.Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Science,Haikou 571101;2.Key Laboratory for Agro-Biotechnology in College of Life Science,Shihezi University,Shihezi 832003;3.Institute of Biological Application,Xinjiang Academy of Agricultural Science,Urumchi 830091 |
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| Abstract: | Objective] The aim of this study was to investigate the prokaryotic expression of pseudomonas aeruginosa Lipose gene. Method] Lipase gene was amplified by PCR from the genome DNA of pseudomonas aeruginosa, and its nucleotide sequence was determined. The prokaryotie expression vector of Lipase gene was constructed by the gene recombination technique. The protein expression was induced for 4 hours by IPTC, with the final concentration of 1.0 mmol/L, and then SDS-PAGE electrophoresis was analyzed. Result] The sequence of mature peptides in Lipase gene cloned from pseudomonas aeroginosa had a 99.36% homology with that of pseudomonas aeruginosa lipase submitted in NCBI, so the prokaryotic expression vector of Lipase gene pET32a-Lip was successfully constructed. Furthermore, the results of SDS-PAGE electrophoresis showed that the target gene was expressed highly and effec-tively. Conclusion] The cloned pseudomonas aeruginosa lipase with its signal peptide could be normally expressed in E. coli and also used for further study. |
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| Keywords: | Pseudomonas aeruginosa Lipase Prokaryotic expression |
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