高效液相色谱法测定尼卡巴嗪含量的方法建立

为了建立高效液相色谱法测定尼卡巴嗪的含量，采用WatersX—BridgeC18（150mm×4．6mm，5μm）色谱柱，乙腈-水（用磷酸调pH至3．0）为流动相，梯度洗脱，流速为1．0mL．min^-1．检测波长0～5min为297nm，5min开始为350nm，柱温30℃，进样体积为20μL。2-羟基-4．6-二甲基嘧啶（HDP）的回归方程为：Y=23452x-456，相关系数r=0．99999，4，4’-二硝基均二苯脲（DNC）的回归方程为：Y=102388x＋5296，相关系数r=0．9999，即HDP在0．29-29．12μg/mL范围内具有良好的线性，DNC在0．71～70．90μg／mL范围内具有良好的线性。该方法简便、准确、灵敏度高、重复性好，可以通过同时测定尼卡巴嗪中HDP和DNC来计算尼卡巴嗪的含量。

Study on the Determination of Nicarbazine by HPLC

To develop the method of HPLC for determination of nicarbazine. The liquid chromatography separation was carried on XBridge C18 column （ 150 mm×4.6 mm,5 μm） with acetonitrile-water（pH was adjusted to 3.0 by H3PQ ） as mobile phase under the column temperature 30 ℃ .The detection wavelength was 297 nm at 0 -5 min, and 350 nm after 5 min, and the injection quantity was 20 μL . The equation of HDP was y = 23452x - 456, r=0.99999; The equation of DNC was y = 102388x ＋ 5296,r=0.9999, the linear range of HDP and DNC were 0.29-29.12 μg/mL and 0.71-70.90 μg/mL respectively. The method was simple , accuracy, and with high sensitivity.The content of nicarbazine can be calculated by determining HDP and DNC at the same time.