| 低能离子修饰技术在L-乳酸菌诱变中的应用 |
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| 引用本文： | 李市场,葛春梅,古绍斌,潘仁瑞,余增亮.低能离子修饰技术在L-乳酸菌诱变中的应用[J].安徽农业科学,2007,35(15):4397-4399. |
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| 作者姓名： | 李市场 葛春梅 古绍斌 潘仁瑞 余增亮 |
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| 作者单位： | 河南科技大学食品与生物工程学院,河南洛阳,471003;合肥学院生物与环境工程系,安徽合肥,230031;中国科学院等离子体物理研究所离子束生物工程学重点实验室,安徽合肥,230031 |
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| 基金项目： | 河南科技大学科学研究基金（2006QN007）. |
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| 摘 要： | 采用离子修饰技术对一株产L-乳酸的米根霉菌P988进行诱变处理。结果表明,在离子注入能量为10 keV,剂量为50×2.6×1013ions/cm2时,诱变效果较好,从正变菌株中反复筛选,得到一株产酸量高的菌株PN2207,产酸量达110 g/L,比出发菌株提高了23%,经过连续传代试验,其遗传性状稳定;并对其发酵条件如碳氮源和CaCO3进行研究,在含糖150 g/L的摇瓶中发酵,其L-乳酸产量达110 g/L。
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| 关 键 词： | 离子注入 L-乳酸 米根霉 |
| 文章编号： | 0517-6611（2007）15-04397-03 |
| 修稿时间： | 2007-02-26 |
Breeding of the Strains with High Yield of L-lactic Acid with Ion Implantation |
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| LI Shi-chang, et al.Breeding of the Strains with High Yield of L-lactic Acid with Ion Implantation[J].Journal of Anhui Agricultural Sciences,2007,35(15):4397-4399. |
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| Authors: | LI Shi-chang |
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| Abstract: | A study on Rhizopus oryzae P998 with the production of L-lactic acid with the technology of ion implantation was made.The result showed that the optimal mutagenic effect was at the ion dose of 50×2.6×1013 ions/cm2 and the energy of 10 keV.After lots of screening,a mutant strain PN2207 was obtained whose activity of L-lactic acid about was 23% higher than that of the original strain P998.And it was found that the yield of L-lactic acid from the strain PN2207 was rather stable through successive transfer of cultures.And the optimal carbon and nitrogen source,CaCO3 and their optimal sugar concentration were determined.The concentration of L-lactic acid reached to 110 g/L under the condition of 150 g/L glucose in fermentation medium. |
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| Keywords: | Ion implantation L-lactic acid Rhizopus oryzae |
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