番茄花叶病毒番茄分离物与烟草花叶病毒蚕豆分离物生物学、血清学比较及PCR特异性检测

 对番茄花叶病毒番茄分离物(ToMV-To-S1)和烟草花叶病毒蚕豆分离物(TMV-B935A)进行了生物学和血清学比较。人工接种6科20种植物,这两个分离物均能侵染其中的3科14种植物,但在普通烟(白肋烟、黄苗榆)上的症状差异较大,前者在这些植物上引起局部症状,后者为系统症状。在琼脂双扩散试验中,两者与ToMV-To-S1抗血清和4种TMV抗血清均形成沉淀线,但沉淀线之间产生刺角,说明ToMV-To-S1和TMV-B935A虽有血清关系,但亦有差异。胶内交叉吸附试验也证明两者之间有血清学差异。根据已报道的ToMV-L株系和TMVU1株系CP基因的3'端非编码区序列设计了ToMV和TMV特异引物,采用PCR方法,这些引物能够特异性的检测区分ToMV-To-S1和TMV-B935A。

BIOLOGY AND SEROLOGICAL COMPARISON OF ToMV TOMATO ISOLATE AND TMV BROAD BEAN ISOLATE AND SPECIFIC DETECTION WITH POLYMERASE CHAIN REACTION

Biology and serological comparison between ToMV tomato isolate (ToMV-To-S1) and TMV broad bean isolate (TMV-B935A) was made. Both ToMV-To-S1 and TMV-B935A could infected 14 plant species in 3 families when 20 plant species in 6 families were tested. The difference was found in Nicotiana tabacum (N. tabacum-White Burley, N. tabacum-Huang Miaoyu), ToMV-To-S1 induced local lesions in these plant species, while TMV-B935A produced systemic mosaic symptoms. In double-diffusion tests, spur formation was observed between ToMV-To-S1 and TMV-B935A, and the results of intragel cross-absorption tests agreed with the doublediffusion tests results. Specific primers corresponding to the coat protein gene and 3' non coding region were designed. When ToMV-To-S1 cDNA templete were used, a specific band could be amplified with ToMV primers by PCR, No band was found when we used TMV primers. With TMV-B935A templete, only TMV primers could produce a specific band. So it is easy to distinguish ToMV-To-S1 and TMV-B935A with ToMV and TMV specific primers.