| 红掌组培过程中外植体褐化问题的解决措施 |
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| 引用本文： | 祁翔,刘燕,王莹,王济红.红掌组培过程中外植体褐化问题的解决措施[J].贵州农业科学,2010,38(2). |
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| 作者姓名： | 祁翔 刘燕 王莹 王济红 |
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| 作者单位： | 贵州省生物研究所,贵州,贵阳,550009 |
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| 基金项目： | 贵州科学院青年基金项目“热带花卉红掌的引种栽培及组培育苗技术研究”[院科合基字(2006)013] |
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| 摘 要： | 为解决红掌组培过程中外植体褐化影响组培成活的问题,通过对外植体采用不同消毒方法处理、对培养基进行改良、调节和对培养条件选择等方面进行研究,发现:红掌在接种前先用抗氧化剂处理和在培养基中添加相应的抗氧化剂有助于减少外植体的褐化,从而为提高红掌的组培繁殖率奠定基础。
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| 关 键 词： | 红掌 外植体 褐化 抗氧化剂 |
Measures to Solve the Browning of Explants in Tissue Culture of Anthurium andraeanum |
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| QI Xiang,LIU Yan,WANG Ying,WANG Ji-hong.Measures to Solve the Browning of Explants in Tissue Culture of Anthurium andraeanum[J].Guizhou Agricultural Sciences,2010,38(2). |
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| Authors: | QI Xiang LIU Yan WANG Ying WANG Ji-hong |
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| Institution: | Guizhou Institute of Biology;Guiyang;Guizhou 550009;China |
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| Abstract: | Different disinfection methods were applied to explants,improvement and adjustment of culture media,and culture conditions selection were studied in this paper to solve the browning of explants which affected the survive of Anthurium andreanum in tissue culture.Results showed that pretreated by anti-oxidant before transplanting and corresponding oxidants added in culture media could reduce the browning,sequentially the reproduction rate of explants could be enhanced. |
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| Keywords: | Anthurium andraeanum explants browning anti-oxidant |
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