| 青花菜高效离体再生体系的建立 |
| |
| 引用本文： | 秦耀国,雷建军,曹必好,李焕秀,谢文华.青花菜高效离体再生体系的建立[J].西南农业大学学报,2005,27(5):653-656. |
| |
| 作者姓名： | 秦耀国 雷建军 曹必好 李焕秀 谢文华 |
| |
| 作者单位： | [1]四川农业大学林学园艺学院,四川雅安625014 [2]华南农业大学园艺学院,广东广州510642 |
| |
| 基金项目： | 广东省重大专项资助项目（2002 A 2070301） |
| |
| 摘 要： | 以青花菜为试验材料，探讨了影响离体再生的一些因素，结果表明：取用4～5d龄无菌苗的带柄子叶和下胚轴，分别培养在MSB＋NAA0．2mg／L＋6-BA2mg／L和MSB＋NAA0．2mg／L＋6-BA1．0mg／L＋AgNO3 2．5mg／L培养基上，获得了较高的分化率（80％）；再生芽插入MS＋IBA0．2mg／L培养基，生根率达100％。
|
| 关 键 词： | 青花菜 再生 分化 AgNO3 |
| 文章编号： | 1000-2642（2005）05-0653-04 |
| 收稿时间： | 2004-12-06 |
| 修稿时间： | 2004-12-06 |
ESTABLISHMENT OF A HIGHLY EFFICIENT IN VITRO REGENERATION SYSTEM FOR BROCCOLI |
| |
| QIN Yao - guo , LEI Jian - jan , CAO Bi - hao, LI Huan - xiu , XIE Wen - hua.ESTABLISHMENT OF A HIGHLY EFFICIENT IN VITRO REGENERATION SYSTEM FOR BROCCOLI[J].Journal of Southwest Agricultural University,2005,27(5):653-656. |
| |
| Authors: | QIN Yao - guo LEI Jian - jan CAO Bi - hao LI Huan - xiu XIE Wen - hua |
| |
| Institution: | 1. College of Forestry and Horticulture, Sichuan Agricultural University, Yaan, Sichuan 625014, China; 2. College of Horticulture, South China Agricultural University, Guangzhou, Guangdong 510642, China |
| |
| Abstract: | Cotyledons w ith petiole and hypocotyls of 4-to 5-day-old virus-free seed lings of broccoli(Brassica oleraceavar.italica) were used as exp lants inin vitrocu lture.Fairly h igh shoot d ifferentiation rate(>80%) was obtained when cotyledons were cu ltured on MSB + NAA 0.2 mg /L + 6-BA 2 mg /L and hypocotyls were cu ltured on MSB + NAA 0.2 mg /L + 6-BA 1.0 mg /L + AgNO3 2.5 mg /L.A ll the shoots rooted read ily when transferred toMS + IBA 0.2 mg /L. |
| |
| Keywords: | broccoli regeneration differentiation AgNO3 |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《西南农业大学学报》浏览原始摘要信息 |
| 点击此处可从《西南农业大学学报》下载免费的PDF全文 |
