饲料中4种硝基呋喃类药物的检测方法研究

建立了饲料中呋喃唑酮、呋喃它酮、呋喃西林和呋喃妥因4种药物的液相色谱分析方法。液相色谱柱为Waters SunfireTMC18(150 mm×2.1 mm,3.5μm),柱温30℃,流动相为乙腈和0.2%甲酸水溶液,检测波长365 nm,流速0.2 mL/min。此条件下硝基呋喃类药物的线性范围是0.1~10μg/mL,回收率为75%~90%,变异系数为3.24%~5.83%。检测限为0.5 mg/kg,定量限为1.0 mg/kg。

Determination of Furazolidone,Furaltadone,Nitrofurazone,Nitrofurantoin in Feed Using HPLC

A HPLC method for determination of furazolidone,furaltadone,nitrofurazone and nitrofurantoin in feed was established.The HPLC apparatus was assembled Waters Sunfire C18 column(150 mm×2.1 mm,3.5 μm),detection wavelength was at 365 nm.Aacetonitril and 0.2% formic acid in water was used as mobile phase with a flow rate of 0.2 mL/min.The linearity range of all nitrofran drugs was 0.1~10 μg/mL.The reclaim ratio was 75%~90%.The variation coefficient was 3.74%~5.83%.The limit of detection was 0.5 mg/kg and the lim...