| 喉癌细胞酵母双杂交cDNA文库的构建 |
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| 引用本文： | 刘雪晴,王春苗,田颖新,贾天军.喉癌细胞酵母双杂交cDNA文库的构建[J].河北北方学院学报(自然科学版),2012(1):59-62,66. |
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| 作者姓名： | 刘雪晴 王春苗 田颖新 贾天军 |
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| 作者单位： | 河北北方学院病原生物与免疫学研究所 |
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| 摘 要： | 目的构建喉癌(Hep-2)细胞的酵母双杂交cDNA文库,为筛选包涵体膜蛋白分子的作用配体提供前期工作基础.方法从Hep-2细胞中提取总RNA,应用SMARTTM技术,构建以pGADT7-Rec为载体的Hep-2细胞酵母GAL4AD融合cDNA文库.结果所构建的文库展现出良好的多态性.结论可用于酵母双杂交系统的筛选.
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| 关 键 词： | Hep-2细胞 cDNA文库 酵母双杂交 |
Construction of Yeast Two-hybrid cDNA Library for Hep-2 Cells |
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| Institution: | LIU Xue-qing,WANG Chun-miao,TIAN Ying-xin,et al(Pathogeny Biology and Immunology Research Institute,Hebei North University,Zhangjiakou 075000,Hebei,China) |
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| Abstract: | Objective To construct yeast two-hybrid cDNA library for Hep-2 cells to screen GAL4 fusion,and provide foundation for screening ligands of Chlamydia Inclusion membrane proteins.Methods The yeast GAL4 fusion cDNA library from Hep-2 cells was constructed.Total RNA of Hep-2 cells was purified by Trizol reagents,and cDNAs of the mRNAs contained in purified total RNA sample were synthesized by SMARTTM technology,which were subsequently cloned into plasmid pGADT7-Rec.Results The library showed excellent polymorphism.Conclusion The constructed library could be used for yeast two-hybrid system. |
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| Keywords: | Hep-2 cells cDNA library Yeast two-hybrid |
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