| 不同产地白鲜皮中黄柏酮含量测定及聚类分析 |
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| 引用本文： | 周丽丽,左玲,马冬磊.不同产地白鲜皮中黄柏酮含量测定及聚类分析[J].河北北方学院学报(自然科学版),2012(1):12-14,20. |
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| 作者姓名： | 周丽丽 左玲 马冬磊 |
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| 作者单位： | 安徽科技学院 |
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| 基金项目： | 安徽科技学院青年科研基金项目:滁州地区白鲜皮的生物学特性及引种驯化研究(ZRC2011282);安徽科技学院大学生创新课题(滁州白鲜成分提取及产地加工工艺研究) |
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| 摘 要： | 目的测定不同产地白鲜皮中黄柏酮含量并进行聚类分析.方法采用HPLC法测定不同产地白鲜皮中黄柏酮含量,色谱柱:ZORBAX SB-C18(4.6mm×150mm,5μm);流动相:甲醇-水(60∶40);检测波长:黄柏酮215nm;柱温:40℃;流速:1mL.min-1;进样量:10μL;记录时间:20min.然后采用计算机的模式识别方法进行聚类分析.结果不同产地白鲜皮药材中黄柏酮的含量分别为:辽宁0.151 6%,内蒙古0.234 8%,黑龙江0.149 9%,吉林0.150 8%,河北0.194 6%,陕西0.159 8%,山东0.162 1%,安徽0.210 2%.范围为1.499～2.348mg/g.结论不同产地白鲜皮中黄柏酮含量不尽相同、东北部地区大致可归为一类.
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| 关 键 词： | 白鲜皮 HPLC 黄柏酮 含量测定 聚类 |
Determination and Hierachical Cluster Analysis of Obacunone in Cortex Dictamni in Different Areas |
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| ZHOU Li-li,ZUO Ling,MA Dong-lei.Determination and Hierachical Cluster Analysis of Obacunone in Cortex Dictamni in Different Areas[J].Journa of Hebei North University:Natural Science Edition,2012(1):12-14,20. |
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| Authors: | ZHOU Li-li ZUO Ling MA Dong-lei |
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| Institution: | (Anhui Science and Technology University,Fengyang 233100,Anhui,China) |
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| Abstract: | Objective To determine obacunone in Cortex Dictamni in different areas and to conduct hierachical cluster analysis.Methods HPLC determination of Cork ketone content in Densefruit Pittany Root-bark of different areas,Chromatographic column: ZORBAX SB-C18(4.6 mm×150 mm,5 μm),Mobile phase: methanol-water(60∶40);Detected wavelength: Obacunone 215 nm;Column temperature: 40 ℃;Velocity:1mL,min-1;Sample size: 10 μL;Recorded time: 20 min.Pattern recognition method of computer was used to conduct hierachical cluster analysis.Result The contents of obacunone in Cortex Dictamni in different areas were:Liaoning 0.1516%,Neimeng 0.2348%,Heilongjiang 0.1499%,Jilin 0.1508%,Hebei 0.1946%,Shanxi 0.1598%,Shandong 0.1621%,and Anhui 0.2102%.The range was 1.499~2.348mg/g.Conclusion Cortex Dictamni in different areas contains diffrerent amount of obacunone.The samples of northeast region can be classified into same category. |
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| Keywords: | Cortex Dictamni HPLC obacunone determination hierachical clusteranalysis |
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