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| 内蒙古3个绒山羊RAPD的初步研究 | |
| 引用本文： | 尹俊,李金泉,李玉荣,赵胜军,赖双英.内蒙古3个绒山羊RAPD的初步研究[J].内蒙古农业大学学报(自然科学版),2001,22(2):44-47. |
| 作者姓名： | 尹俊 李金泉 李玉荣 赵胜军 赖双英 |
| 作者单位： | 1. 内蒙古农业大学生物工程系，呼和浩特 010018 2. 内蒙古畜牧科学院，呼和浩特 010000 3. 内蒙古农业大学动物科技学院，呼和浩特 010018 |
| 基金项目： | 国家自然科学地区重点基金，内蒙古科技厅联合资助! (资助号 :399690 0 2 ) |
| 摘 要： | 利用随机扩增多态DNA（RAPD）技术，研究了内蒙古地区3个绒山羊品种的DNA多态性，由此推导它们之间的遗传距离和系统发育关系。在所使用的12种随机引物中，有8种随机引物扩增出多态谱带，共检测到32个RAPD标记，其中有21个发生变异。用Nei的片段公享度公式计算了品种间的遗传距离，用UPGMA聚类法构建了系统发育树。结果表明：内蒙古白绒山羊的3个类群彼此亲缘关系较近，分化不明显；内蒙古白绒山羊与罕山白绒山羊聚为一个大类，辽宁绒山羊和乌珠穆泌白绒山羊聚为一个大类，说明内蒙古白绒山羊与罕山白绒山羊有较近的亲缘关系，乌珠穆沁白绒山羊与辽宁绒山羊的亲缘关系较近。 |
| 关 键 词： | 白绒山羊 遗传距离 亲缘关系 品种 RAPD |
| 文章编号： | 1009-3575(2001)02-0044-04 |
| 修稿时间： | 2001年3月15日 |
GENETIC DIVERSITY DETECTED BY RAPD IN THREE INNER MONGOLIA CASHMERE GOAT BREEDS |
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| YIN Jun ,LI Jin-quan ,LI YU-rong ,ZHAO Sheng-jun ,LAI Shuang-ying.GENETIC DIVERSITY DETECTED BY RAPD IN THREE INNER MONGOLIA CASHMERE GOAT BREEDS[J].Journal of Inner Mongolia Agricultural University(Natural Science Edition),2001,22(2):44-47. | |
| Authors: | YIN Jun LI Jin-quan LI YU-rong ZHAO Sheng-jun LAI Shuang-ying |
| Institution: | YIN Jun 1,LI Jin-quan 1,LI YU-rong 2,ZHAO Sheng-jun 3,LAI Shuang-ying 3 |
| Abstract: | Random amplified polymorphic DNA(RAPD) analysis was used to estimate genetic diversity and relationship in 6 DNA pools which belong to three Inner Mongolia cashmerc goat breeds,12 primers were used,among which 8 generated polymorphic bands.32 RAPD markers were marked,of which 21 were polymorphic marker.The genetic distance matrix was obtained by proportion of shared fragments.Dendrogram was generated by UPGMA.The results indicated that Inner Mongolia cashmere goat strains' phylogenetic relations were close;Inner Mongolia cashmere goat and Hanshan were clustered together;Liaoning cashmere goat was closely related to Wuzhumuqin. |
| Keywords: | cashmere goat genetic structure RAPD |
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