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Harpin_(Ea)激发烟草过敏性反应与过氧化物酶和多酚氧化酶活力关系的研究
引用本文:乔卿梅,程茂高,蒋士君,王军红.Harpin_(Ea)激发烟草过敏性反应与过氧化物酶和多酚氧化酶活力关系的研究[J].河南农业科学,2008(1):30-32,36.
作者姓名:乔卿梅  程茂高  蒋士君  王军红
作者单位:1. 郑州牧业工程高等专科学校,生物工程系,河南,郑州,450011
2. 河南农业大学,植物保护学院,河南,郑州,450002
基金项目:河南省教育厅科技攻关项目(200130465)
摘    要:HarpinEa是植物病原细菌Erwinia amylovora分泌的一种蛋白质类激发子,能激发烟草的过敏性反应。用30μg/mL的HarpinEa溶液注射到具有不同抗感能力的烟草品种中,测定不同品种的过氧化物酶(POD)和多酚氧化酶(PPO)的活力,结果显示:不同抗感能力烟草品种的POD,PPO活力变化在时间上均存在一致性;抗病品种的POD和PPO活力高于感病品种。说明烟草体内的POD,PPO活力变化规律同烟草品种的抗感性在一定程度上呈正相关关系。

关 键 词:HarpinEa  烟草  过氧化物酶  多酚氧化酶
文章编号:1004-3268(2008)01-0030-04
收稿时间:2007-08-10
修稿时间:2007年8月10日

Relations of HarpinEa-excited Hypersensitive Response with Peroxidase and Polyphenol-oxidase in Tobacco Leaves
QIAO Qing-mei,CHENG Mao-gao,JIANG Shi-jun,WANG Jun-hong.Relations of HarpinEa-excited Hypersensitive Response with Peroxidase and Polyphenol-oxidase in Tobacco Leaves[J].Journal of Henan Agricultural Sciences,2008(1):30-32,36.
Authors:QIAO Qing-mei  CHENG Mao-gao  JIANG Shi-jun  WANG Jun-hong
Abstract:HarpinEa can excitated the hypersensitive response of tobacco leaves.30μg/mL HarpinEa of solution was injected into the tobacco varieties with different resistance to diseases,and their activities of Peroxidase(POD) and Polyphenol-oxidase(PPO) were measured.The result showed that the activity changes of POD and PPO were consistent in time for all the varieties,but the activities of POD and PPO were higher in resistant varieties than in susceptible ones.This indicated that there existed positive correlation between the change disciplinarian of POD and PPO and the disease resistence of tobacco varieties to a certain extent.
Keywords:HarpinEa  Tobacco  Peroxidase  Polyphenol-oxidase
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