| 影响Fat-1转基因水牛胚胎发育因素的研究 |
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| 引用本文： | 王建,庞春英,邓廷贤,杨春艳,郑海英,黄芬香,杨炳壮,梁贤威.影响Fat-1转基因水牛胚胎发育因素的研究[J].中国畜牧兽医,2012,39(12):121-124. |
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| 作者姓名： | 王建 庞春英 邓廷贤 杨春艳 郑海英 黄芬香 杨炳壮 梁贤威 |
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| 作者单位： | 中国农业科学院广西水牛研究所, 农业部(广西)水牛遗传繁育重点实验室,广西南宁 530001 |
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| 基金项目： | 桂科基2010GXNSFA013096，桂科攻1123005-1，"广西'特聘专家'专项经费资助" |
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| 摘 要： | 本试验比较了水牛卵母细胞体外受精后注射外源DNA的时间(5~6、9~10、13~14、17~18、21~22 h)和外源DNA的浓度(10、50、100 μg/mL)对Fat-1转基因水牛胚胎的发育影响。结果显示,9~10 h组的胚胎表达率和囊胚表达率最高,其中胚胎表达率极显著高于5~6 h组,囊胚表达率极显著高于21~22 h组(P<0.01);在浓度试验中,50 μg/mL浓度组的胚胎表达率和囊胚表达率最高,且极显著高于100 μg/mL(P<0.01)。
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| 关 键 词： | 水牛 转基因 Fat-1 胚胎 |
| 收稿时间： | 2012-05-09 |
Study on Factors Affecting the Development of Fat-1 Buffalo Transgenic Embryo |
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| WANG Jian
,
PANG Chun-ying
,
DENG Ting-xian
,
YANG Chun-yan
,
ZHENG Hai-ying
,
HUANG Fen-xiang
,
YANG Bing-zhuang
,
LIANG Xian-wei.Study on Factors Affecting the Development of Fat-1 Buffalo Transgenic Embryo[J].China Animal Husbandry & Veterinary Medicine,2012,39(12):121-124. |
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| Authors: | WANG Jian PANG Chun-ying DENG Ting-xian YANG Chun-yan ZHENG Hai-ying HUANG Fen-xiang YANG Bing-zhuang LIANG Xian-wei |
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| Institution: | Key Laboratory of Buffalo Genetics, Breeding and Reproduction Technology, Ministry of Agriculture (Guangxi), Guangxi Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning 530001, China |
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| Abstract: | The effects of injecting timing after fertilization (5 to 6, 9 to 10, 13 to 14, 17 to 18, 21 to 22 h) and exogenous DNA concentrations (10, 50 and 100 μg/mL) on the development of Fat-1 transgenic embryo were investigated. The results showed that the EGFP expressing rate and blastocysts’ EGFP expressing rate of 9 to 10 h group were the highest, the EGFP expressing rate were significantly higher than 5 to 6 h group(P<0.01) and the blastocysts’ EGFP expressing rate significantly higher than 21 to 22 h group(P<0.01);in the concentration test, the EGFP expressing rate and blastocysts’ EGFP expressing rate of 50 μg/mL group were the highest, and significantly higher than 100 μg/mL(P<0.01). |
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| Keywords: | buffalo transgenic Fat-1 embryos |
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