| pCAMBIA2300-betA-BADH双价基因植物表达载体的构建 |
| |
| 引用本文： | 咸 洋,夏 阳,张金文,庞彩红,李 丽,毛秀红.pCAMBIA2300-betA-BADH双价基因植物表达载体的构建[J].中国农学通报,2009,25(9):47-50. |
| |
| 作者姓名： | 咸 洋 夏 阳 张金文 庞彩红 李 丽 毛秀红 |
| |
| 作者单位： | 咸洋,Xian Yang(甘肃农业大学生命科学技术学院,兰州,730070;山东省林业科学研究院,济南,250014);夏阳,庞彩红,李丽,毛秀红,Xia Yang,Pang Caihong,Li Li,Mao Xiuhong(山东省林业科学研究院,济南,250014);张金文,Zhang Jinwen(甘肃农业大学生命科学技术学院,兰州,730070)
|
| |
| 摘 要： | 摘要：本实验以载体pCAMBIA2300-35s-OCS为基础通过分子克隆手段，将甜菜碱合成途径的两个关键酶基因，即编码胆碱脱氢酶（CDH）的betA基因和编码甜菜碱醛脱氧酶（BADH）的BADH基因及启动子和终止序列构建在同一植物表达载体上，得到pCAMBIA2300-betA-BADH双价植物表达载体。并将其导入根癌农杆菌GV3101和LBA4404，进一步用于双子叶植物的遗传转化。
|
| 关 键 词： | 茶树 茶树 生长量 拟合公式 |
| 收稿时间： | 2009-01-23 |
| 修稿时间： | 2009-02-11 |
Construction of plant expression vector of pCAMBIA2300-betA-BADH |
| |
| Xian Yang,Xia Yang,Zhang Jinwen,Pang Caihong,Li Li,Mao Xiuhong.Construction of plant expression vector of pCAMBIA2300-betA-BADH[J].Chinese Agricultural Science Bulletin,2009,25(9):47-50. |
| |
| Authors: | Xian Yang Xia Yang Zhang Jinwen Pang Caihong Li Li Mao Xiuhong |
| |
| Institution: | Xian Yang, Xia Yang, Zhang Jinwen, Pang Caihong, Li Li, Mao Xiuhong (1College of Life Science and Technology, Gansu Agriultural Univesity, Lanzhou 730070; 2Shandong Academy of Forestry, Jinan 250014) |
| |
| Abstract: | Abstract: The choline dehydrogenase（CDH）gene and the betaine aldehyde dehydrogenase（BADH）gene are the key genes of the glycine betaine’s synthesis pathway. They were cloned into the vector pCAMBIA2300-35s-OCS.They were tandem and have their respective promoters and terminators. The vector pCAMBIA2300-betA-BADH was transformed into the Agrobactrium tumefaciens GV3101, LBA4404 and will be used in the genetic transformation of the dicotyledons. |
| |
| Keywords: | beta BADH PCR |
| 本文献已被 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国农学通报》浏览原始摘要信息 |
| 点击此处可从《中国农学通报》下载免费的PDF全文 |
|
