| 东方百合四倍体及2 n雌配子杂种后代RAPD分析 |
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| 引用本文： | 郑思乡,吴福川,向仕华,章海龙,张力,黄静.东方百合四倍体及2 n雌配子杂种后代RAPD分析[J].西南农业大学学报,2005,27(4):515-517. |
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| 作者姓名： | 郑思乡 吴福川 向仕华 章海龙 张力 黄静 |
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| 作者单位： | [1]云南玉溪雷森科技有限公司,云南玉溪650031 [2]云南农业大学,园林园艺学院,云南昆明650201 |
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| 基金项目： | 云南雷森科技有限公司资助项目：东方百合多倍体研究 |
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| 摘 要： | 对组培条件下秋水仙素诱变的东方百合四倍体及2 n雌配子杂种后代进行了RAPD分析.结果表明：东方百合四倍体诱导过程中,少数植株除了染色体数目加倍以外,还存在DNA序列的改变;2 n雌配子杂种后代RAPD分析表现出丰富的遗传多样性,少数植株RAPD分析差异巨大,特别是T8无性后代存在较大的性状差异,个别植株经RAPD分析与其他单株存在较大的差异.
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| 关 键 词： | 东方百合 2 n雌配子 多倍体 RAPD |
| 文章编号： | 1000-2642（2005）04-0515-03 |
| 收稿时间： | 2004-10-18 |
| 修稿时间： | 2004-10-18 |
RAPD ANALYSIS OF THE TETRAPLOID AND 2 n FEMALE GAMETE HYBRID OFFSPRINGS OF LILIUM ORIENTAL |
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| ZHENG Si - xiang WU Fu - chuan, XIANG Shi - hua, ZHANG Hai - long, ZHANG Li, HUANG Jing.RAPD ANALYSIS OF THE TETRAPLOID AND 2 n FEMALE GAMETE HYBRID OFFSPRINGS OF LILIUM ORIENTAL[J].Journal of Southwest Agricultural University,2005,27(4):515-517. |
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| Authors: | ZHENG Si - xiang WU Fu - chuan XIANG Shi - hua ZHANG Hai - long ZHANG Li HUANG Jing |
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| Institution: | ZHENG Si - xiang WU Fu - chuan, XIANG Shi - hua, ZHANG Hai - long, ZHANG Li, HUANG Jing( 1. Yunnan Yuxi Leisen Sci |
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| Abstract: | RAPD analysis was made of the colchicine - induced tetraploids of Lilium oriental and the hybrid offspring of its 2 n female gametes. The results showed that DNA sequences changed during polyploidization in a few plants besides the doubling of chromosomes. Abundant genetic diversities were observed in the 2 n female gamete offspring. Substantial differences were observed in the asexually propagated offspring of T8 in their characters. Some individuals were shown to be considerably different from others in the RAPD analysis. |
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| Keywords: | Lilium oriental 2 n female gamete polyploid RAPD |
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