Teleost liver hexokinase- and glucokinase-like enzymes: partial cDNA cloning and phylogenetic studies in rainbow trout (Oncorhynchus mykiss), common carp (Cyprinus carpio) and gilthead seabream (Sparus aurata)+ |
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Authors: | C Blin S Panserat F Médale E Gomes J Brèque S Kaushik R Krishnamoorthy |
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Institution: | (1) INSERM U458, H^opital Robert Debré, 75019 Paris, France;(2) Laboratory of Fish Nutrition, INRA-IFREMER, 64310 St-Pée-sur-Nivelle, France;(3) Instituto Ciencas Biomedicas Abel Salazar, Universidade do Porto, 4000 Porto, Portugal |
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Abstract: | The efficiency of enzymatic conversion of glucose into glucose-6-phosphate in fish fed carbohydrates is still a matter of debate. This study reports the first ever identification of hexokinase-family members at a molecular level in fish. The deduced amino acid sequences of six cDNA clones allowed their assignement either to hexokinase IV (glucokinase) for rainbow trout (Oncorhynchus mykiss), common carp (Cyprinus carpio) and gilthead seabream (Sparus aurata) or to hexokinase I/II for the common carp (two clones) and the gilthead seabream, based on their similarities to known members of the HK family. The evolutionary relationships among these sequences were investigated by phylogenetic analysis. These hexokinase sequences constitute potential molecular tools for analysis of interspecific differences in dietary carbohydrate utilization in fish. |
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Keywords: | carbohydrate metabolism glucokinase hexokinase phylogenetic studies teleost |
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