| 大白菜RAPD反应条件的优化 |
| |
| 引用本文： | 刘天文,王翠花,于占东,徐文玲,牟晋华,杨晓红.大白菜RAPD反应条件的优化[J].山东农业科学,2008(9). |
| |
| 作者姓名： | 刘天文 王翠花 于占东 徐文玲 牟晋华 杨晓红 |
| |
| 作者单位： | 1. 吉林农业大学,吉林,长春,130000
2. 山东省农业科学院蔬菜研究所,山东,济南,250100 |
| |
| 基金项目： | 国家高新技术研究发展计划(2006AA100108-3-1)项目
|
| |
| 摘 要： | RAPD是作物主要农艺性状分子标记及遗传多样性研究的一项重要技术。为了适应大白菜反应体系,本试验以大白菜品种413、96及其F1为试材,对影响RAPD扩增的模板DNA用量、Mg2+浓度、TaqDNA聚合酶浓度、dNTPs浓度、引物浓度等因素进行了优化,结果如下:20μl PCR反应体系,30 ng(1.5μl)DNA模板,3 mmol/L Mg2+,0.01 U/μl Taq DNA聚合酶,0.4 mmol/L dNTPs,0.8μmol/L引物为最佳反应浓度。
|
| 关 键 词： | 大白菜 RAPD 分子标记 |
Optimal Condition for Random Amplify Polymorphous DNA (RAPD) in Chinese Cabbage |
| |
| LIU Tian-wen,WANG Cui-hua,YU Zhan-dong,XU Wen-ling,MU Jin-hua,YANG Xiao-hong.Optimal Condition for Random Amplify Polymorphous DNA (RAPD) in Chinese Cabbage[J].Shandong Agricultural Sciences,2008(9). |
| |
| Authors: | LIU Tian-wen WANG Cui-hua YU Zhan-dong XU Wen-ling MU Jin-hua YANG Xiao-hong |
| |
| Institution: | LIU Tian-wen1,WANG Cui-hua2,YU Zhan-dong1,XU Wen-ling2,MU Jin-hua2,YANG Xiao-hong1 |
| |
| Abstract: | Random Amplify Polymorphous DNA(RAPD) is an important technique for molecular markers and study on genetic diversity.In order to establish the optimized RAPD detection system,the Chinese cabbage cultivars 413,96 and their F1 were used.The concentration of DNA,Mg2+,Taq DNA polymerase and dNTPs were optimized for RAPD reaction.The results showed that the optimal conditions were 30 ng(1.5 μl) DNA,3 mmol/L Mg2+,0.01 U/μl Taq DNA polymerase,0.4 mmo/L dNTP and 0.8 μmol/L primer in a 20 μl RAPD-PCR reaction mixture. |
| |
| Keywords: | RAPD |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
