| 大花蕙兰试管苗玻璃化发生原因及防止技术研究 |
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| 引用本文： | 朱晋云,杨丽萍,许玉娟,杨玉维,贾学森,张效良,温希荣.大花蕙兰试管苗玻璃化发生原因及防止技术研究[J].山西农业科学,2004,32(3):47-50. |
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| 作者姓名： | 朱晋云 杨丽萍 许玉娟 杨玉维 贾学森 张效良 温希荣 |
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| 作者单位： | 1. 山西省农业科学院小麦研究所,山西,临汾,041000
2. 临汾市同世达实业有限公司,山西,临汾,041000 |
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| 摘 要： | 试验结果表明,大花蕙兰试管苗玻璃化的原因主要有3个:一是培养温度的突然升高,造成高温伤害的后遗症,引发玻璃化;二是经过多次继代培养,原球茎过多吸收细胞分裂素6-BA,使体内激素比例严重失调,导致玻璃化;三是长期使用氨、锰、铁、锌等含量较低的培养基,引起玻璃化苗的产生。采取相应的技术措施,可以完全控制玻璃化苗的发生。
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| 关 键 词： | 大花蕙兰 原球茎 试管苗 玻璃化 |
| 文章编号： | 1002-2481(2004)03-0047-04 |
| 修稿时间： | 2003年11月28 |
Study on Vitrifiable Genetic Reasons of Cymbidium Grandiflorium Tube Plantlet and Its Control Technique |
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| ZHU Jin-yun,YANG Li-ping,XU Yu-juan,YANG Yu-wei,JIA Xue-sen,ZHANG Xiao-liang,WEN Xi-rong.Study on Vitrifiable Genetic Reasons of Cymbidium Grandiflorium Tube Plantlet and Its Control Technique[J].Journal of Shanxi Agricultural Sciences,2004,32(3):47-50. |
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| Authors: | ZHU Jin-yun YANG Li-ping XU Yu-juan YANG Yu-wei JIA Xue-sen ZHANG Xiao-liang WEN Xi-rong |
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| Institution: | ZHU Jin-yun~1,YANG Li-ping~1,XU Yu-juan~1,YANG Yu-wei~1,JIA Xue-sen~2,ZHANG Xiao-liang~2,WEN Xi-rong~2 |
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| Abstract: | The present experiments showed that vitrifiable genetic reasons ofcymbidium grandifiorium of tube plantlet had three. Firstly, the culture temperature sudden rise and the high temperature damage caused sequelae of tube plantlet vitrifiation. Secondly,after more subculture, the protocorm derived from Cymbidium grandiflorium absorbed overabundance cytokinin 6-BA (6-benzyladenine),which aroused hormone proportion inbalanc in plant body and generated vitrification. Thirdly, the culture medium which contained lower ammonia, manganese, iron, zinc etc, were longtermly used, and this probably caused vitrification.It concluded that when technical measures were taken,a thorough control of vitrification is possible. |
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| Keywords: | Cymbidium grandiflorium Protocorm Tube plantlet Vitrification |
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