黑芝麻愈伤组织的诱导及分化的初步研究

以黑芝麻(Sesamum indicum DC)成熟种子无菌苗的子叶、子叶节、下胚轴作为外植体,选用MS基本培养基,并设置4种蔗糖浓度作愈伤组织诱导培养基,以2,4-D、6-BA、IAA、KT组成16种生长调节剂浓度配伍的分化培养基,对愈伤组织的形成及分化进行了研究.结果表明,蔗糖浓度30 g/L时对子叶、子叶节愈伤组织诱导较为有利,蔗糖浓度40 g/L时对下胚轴愈伤组织诱导较为有利,分化培养基为MS 6-BA 2.0 mg/L IAA 1.5 mg/L时,子叶愈伤组织的分化率达30.0%,其效果较好.

Study on Callus Inducement and Differentiation of Black Sesame Seed

Cotyledon,cotyledonary node and hypocotyl of aseptic seedling of mature black sesame seed were taken as explants,using MS as basic culture medium.There were four kinds of sucrose concentration medium of callus inducement;MS medium with 6-BA,KT,IAA and 2,4-D were formed up as differential medium of sixteen different growth regulator consistencies,the formation and differentiation of callus were researched.The results showed that 30 g/L-cane sucrose concentration is quite advantageous to the callus inducement of cotyledon and cotyledonary node,40 g/L-cane sucrose concentration is quite advantageous to callus inducement of hypocotyl,and the differential medium of MS+ 6-BA 2.0 mg/L+ IAA 1.5 mg/L can make the differenciation frequency of cotyledon callus reach 30.0%,which is the most effective.