| 绿僵菌不同菌株DNA多态性的RAPD分析 |
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| 引用本文： | 张立钦,林新春,毛胜凤.绿僵菌不同菌株DNA多态性的RAPD分析[J].浙江林学院学报,2001,18(3):281-285. |
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| 作者姓名： | 张立钦 林新春 毛胜凤 |
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| 作者单位： | 浙江林学院资源与环境系, |
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| 基金项目： | 浙江省“151人才”基金资助项目(浙人专1999-27) |
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| 摘 要： | 采用RAPD－PCR技术分析了4个绿僵菌株的DNA遗传多态性。从160条引物中筛选出27条引物，对各菌株进行PCR扩增，结果4个绿僵菌菌株共扩增出334个位点，其中多态性位点299个，占89．5％，表明各菌株间具有较丰富的遗传多态性。菌株DNA多态性与原寄主和孢子形态表现出明显的相关性，但与对松墨天牛幼虫的毒力间未表现出相关性。图2表4参7
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| 关 键 词： | 绿僵菌 分子标记 松墨天牛 菌株 DND 多态性 RAPD |
| 文章编号： | 1000-5692(2001)03-0281-05 |
Analysis on isolates of Metarrhizium anisopliae by RAPD |
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| ZHANG Li qin,LIN Xin chun,MAO Sheng feng.Analysis on isolates of Metarrhizium anisopliae by RAPD[J].Journal of Zhejiang Forestry College,2001,18(3):281-285. |
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| Authors: | ZHANG Li qin LIN Xin chun MAO Sheng feng |
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| Abstract: | DNA of Metarrhizium anisopliae isolates was analyzed by RAPD.Twenty seven primers screened and optimized out of 160 primers were applied to the proliferation of PCR.Three hundred four loci have been proliferated in 4 strains of M. anisopliae,299 loci out of which are of polymorphism and account for 89 5%.All these suggest that each strain has an abundant genetic polyorphism.There is some correlation between DNA polymorphism of strains and their original hosts,size of conidiospore,distributions,but no obvious correlation between DNA polymorphism and virulence on Monochamus alternatus larvae. |
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| Keywords: | Metarrhizium anisopliae random amplified polymorphic DNA molecular warkers Monochamus alternatus |
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