| 决明子叶愈伤组织中大黄素与大黄酚含量的测定 |
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| 引用本文： | 周嘉裕,廖海,李利平,刘乐,贺葵邦,马丽.决明子叶愈伤组织中大黄素与大黄酚含量的测定[J].安徽农业科学,2008,36(21). |
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| 作者姓名： | 周嘉裕 廖海 李利平 刘乐 贺葵邦 马丽 |
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| 作者单位： | 西南交通大学药学院,四川峨眉,614202 |
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| 基金项目： | 西南交通大学校科研和教改项目 |
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| 摘 要： | 目的]通过愈伤组织的诱导培养,提高决明材料中大黄素或大黄酚的含量。方法]用MS培养基萌发决明幼苗、诱导子叶愈伤组织,用HPLC法测定子叶愈伤组织中大黄素、大黄酚的含量。结果]子叶愈伤组织中大黄素含量为0.099%,大黄酚含量为0.132%;决明子中的大黄素含量为0.029%,大黄酚含量为0.190%。结论]诱导子叶愈伤组织的方法有助于提高决明材料中的大黄素含量。
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| 关 键 词： | 决明 愈伤组织 蒽醌 HPLC |
Determination of Emodin and Chrysophanol Contents in Callus of Cassia tora L.Leaves |
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| ZHOU Jia-yu et al.Determination of Emodin and Chrysophanol Contents in Callus of Cassia tora L.Leaves[J].Journal of Anhui Agricultural Sciences,2008,36(21). |
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| Authors: | ZHOU Jia-yu |
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| Abstract: | Objective] Through inductive culture, emodin and chrysophanol contents in cassia seed were increased.Method]MS culture medium was used to bourgeon seedlings of cassia seed and induce cotyledon callus of cassia seed while HPLC method was adopted to determine emodin and chrysophanol contents.Result] Emodin and chrysophanol contents in cotyledon callus were 0.099% and 0.312% respectively while they were 0.029% and 0.190% respectively in cassia seed.Conclusion]The method of inducing cotyledon callus were helpful for increasing emodin content in cassia seed. |
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| Keywords: | HPLC |
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