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复合鱼肉肌原纤维蛋白加热过程中理化特性变化的研究
引用本文:刘蕾,王航,罗永康,宋永令,沈慧星.复合鱼肉肌原纤维蛋白加热过程中理化特性变化的研究[J].淡水渔业,2012,42(3):88-91.
作者姓名:刘蕾  王航  罗永康  宋永令  沈慧星
作者单位:1. 中国农业大学食品科学与营养工程学院,北京,100083
2. 中国农业大学理学院,北京,100083
基金项目:现代农业产业技术体系建设专项资金,国家自然科学基金
摘    要:考察了鲢(Hypophthalmichthys molitrix)、小黄鱼(Pseudosciaena polyactis)及其以质量比1∶1复合的肌原纤维蛋白的浊度、Ca2+-ATPase活性和巯基含量在加热过程中的变化规律。结果显示:随着加热温度的升高,三种蛋白溶液的浊度呈S型曲线增加。复合肌原纤维蛋白的增加模式与小黄鱼肌原纤维蛋白的增加模式相似,不同于鲢肌原纤维蛋白的增加模式。鲢肌原纤维蛋白和小黄鱼肌原纤维蛋白的Ca2+-ATPase活性随温度的升高逐渐下降。复合肌原纤维蛋白的Ca2+-ATPase活性在20~35℃略有上升,而后剧烈下降,55℃时活性完全丧失。复合肌原纤维蛋白的总巯基含量随着加热温度的升高呈下降趋势。当温度在30~60℃时下降缓慢,高于65℃时剧烈下降。

关 键 词:鲢(Hypophthalmichthys  molitrix)  小黄鱼(Pseudosciaena  polyactis)  肌原纤维蛋白  热变性  理化特性

Study on the physicochemical properties of blended fish myofibrillar protein during heat treatment
LIU Lei , WANG Hang , LUO Yong-kang , SONG Yong-ling , SHEN Hui-xing.Study on the physicochemical properties of blended fish myofibrillar protein during heat treatment[J].Freshwater Fisheries,2012,42(3):88-91.
Authors:LIU Lei  WANG Hang  LUO Yong-kang  SONG Yong-ling  SHEN Hui-xing
Institution:1.Food Science and Nutritional Engineering College,China Agricultural University,Beijing 100083,China; 2.College of Science,China Agricultural University,Beijing 100083,China)
Abstract:The study investigated the physiochemical properties of myofibrillar protein from silver carp,yellow croaker and blended myofibrillar protein composed by 50% silver carp protein and 50% yellow croaker protein during heat treatment.As the temperature rose,turbidity of three kinds protein solution increased by S-shape curve.The curve of blended myofibrillar protein is similar to yellow croaker’ s,but is different from the silver carp’ s.As the temperature rose,Ca2+-ATPase activity of the myofibrillar protein from silver carp and yellow croaker gradually decreased.However,Ca2+-ATPase activity of blended myofibrillar protein decreased gradually with a slight increase from 20 ℃ to 35 ℃ and reduced to blank at 55 ℃.Total sulfhydryl group of blended myofibrillar protein began to decrease at 30 ℃ and distinctively dropped when the temperature is higher than 65 ℃.
Keywords:silver carp(Hypophthalmichthys molitrix)  yellow croaker(Pseudosciaena polyactis)  myofibrillar  thermal denaturation  physiochemical properties
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