噁喹酸在欧洲鳗鲡体内的药代动力学及残留研究

在(25±1)℃水温条件下,分别采用口灌和浸浴的给药方式,以20 mg/kg的单剂量混饲口灌及5 mg/L的剂量浸浴18 h给予噁喹酸后,用高效液相色谱法测定血浆和肌肉中的药物浓度,研究了不同给药方式下噁喹酸在欧洲鳗鲡(Anguilla anguilla)体内的药代动力学特征.结果表明:欧洲鳗鲡口灌给药后,其血浆和肌肉...

Pharmacokinetics and residues of oxolinic acid in Anguilla anguilla

At water temperature of(25±1)℃,Anguilla anguilla was administered orally with oxolinic acid(OA) in single dose of 20 mg/kg or by medicated bath in 5 mg/L for 18 hours.The concentration of OA in the plasma and muscle of the eels were determined by high performance liquid chromatography and the pharmacokinetics of OA in eels in different ways of administration were studied.The results showed that after oral administration,the plasma and muscle concentration-time course of OA conformed to a one-compartment open model with the first order absorption.The pharmacokinetic equations were as follows:Cplasma=8.0560(e-0.00181t-e-0.00250t),Cmuscle=2.9347(e-0.00176t-e-0.00217t);after medicated bath,the plasma and muscle concentration-time course of OA both conformed to a two-compartment open model with the first order absorption.The pharmacokinetic equations were as follows,:Cplasma=1.8100e-0.0042t+0.5548 e-0.00143t-2.70 e-0.3007t,Cmuscle=1.2779e-0.0082t+0.0394 e-0.000721t-6.35 e-0.1202t.According to the maximum residue limit(MRL)of 50 μg/kg,the withdrawl period should be no less than 114 days under oral administration and no less than 71days under medicated bath.