HPLC法测定犬血浆及复方麻醉制剂中强痛宁含量的研究

为建立高效液相色谱法(HPLC)-紫外检测器测定犬血浆和复方麻醉制剂(QFM)中强痛宁浓度的方法,本试验采用外标法,以ZorbaxSBC18(4.6mm×250mm,5μm)为固定相,乙腈与去离子水(含0.015mol·L-1的磷酸二氢钠)30:70(v/v)为流动相,流速1.0mL·min-1,紫外检测波长195nm。结果表明,标准曲线在2~100ng·mL-1范围内线性关系良好(r2=0.9975~0.9986),最低检测限浓度为2ng·mL-1,犬血浆和QFM中强痛宁的平均回收率分别为(92.75±3.96)%、(92.50±4.32)%;日内变异系数(4.01±1.38)%、(4.28±1.59)%;日间变异系数(4.60±2.0)%、(44.46±1.87)%。说明本方法简便、准确、检测浓度低,能够满足犬血浆中低浓度强痛宁的测定及临床药代动力学研究的要求。

Research of determining the contents of Qiangtongning in plasma and QFM by HPLC

To determine the concentration of Qiangtongning and Qiangtongning in QFM combination in canine plasma by HPLC with ultraviolet detection, external standard method was used. The eluation of Qiangtongning was carried out by Zorbax SB C18(4.6 mm×250 mm, 5 μm) column with 0.015 mol·L-1 NaH2PO4 acetonitril -H2O (30: 70, v/v) as the mobile phase, and detection wavelength was 195 nm. Standard curve for Qiangtongning was linear in the range of 2-100 ng·mL-1(r=0.9975-0.9986). The detection limit was 2 ng·mL-1. The average recovery of Qiangtongning and Qiangtongning in QFM combination in canine plasma was (92.75 ± 3.96)% and (92.50±4.32)%; the within-day RSD was (4.01±1.38)% and (4.28±1.59)%; the between-day RSD was (4.60±2.0)% and (44.46±1.87)%. This method is quick and accurate for the determination of Qiangtongning in canine plasma and useful for pharmacokinetic study of Qiangtongning.