| Effects of cold stress on hypothalamic corticotrophin-releasing and thyrotropin-releasing hormone messenger RNA levels in chickens |
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| 作者姓名： | WANG Jintao and XU Shiwen* College of Veterinary Medicine Northeast Agricultural University Harbin China Animal Science and Veterinary Medicine Research Institute Heilongjiang Academy of Nongken Sciences Harbin China |
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| 作者单位： | WANG Jintao1，2，and XU Shiwen1* 1 College of Veterinary Medicine，Northeast Agricultural University，Harbin 150030，China 2Animal Science and Veterinary Medicine Research Institute，Heilongjiang Academy of Nongken Sciences，Harbin 150038，China |
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| 摘 要： | There is increasing evidence that stress can activate the hypothalamic-pituitary-adrenal-axis and hypothalamic-pituitary- thyroid-axis,and further affect the synthesis and secretion of corticotrophin-releasing hormone(CRH)and thyrotropin-releasing hormone(TRH).To evaluate the effect of cold stress on the hypothalamic CRH and TRH messenger RNA(mRNA)levels in Yisha chickens,male Yisha chickens were subjected to acute(1,6,12 h)and chronic(5,10,20 d)cold stress(12±1)℃.Hypothalami were collected for assessment of mRNA levels by semi-quantitative RT-PCR.Acute stress resulted in a significant decrease of CRH mRNA levels at 6 and 12 h,and a significant increase of TRH mRNA levels at every stress time point.Chronic cold stress resulted in a significant increase of CRH mRNA levels and a significant decrease of TRH mRNA levels compared with the control group at every stress time point.The results suggest that the two genes differently respond to cold stress at the mRNA levels.And the different degrees of cold stress will produce different effects on the identical gene.
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| 关 键 词： | 鸡 繁殖生理学 下丘脑 冷应力 |
| 文章编号： | 1006-8104（2007）-04-0327-06 |
| 收稿时间： | 2007-03-27 |
Effects of cold stress on hypothalamic corticotrophin-releasing and thyrotropin-releasing hormone messenger RNA levels in chickens |
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| WANG Jintao,,and XU Shiwen* College of Veterinary Medicine,Northeast Agricultural University,Harbin ,China Animal Science and Veterinary Medicine Research Institute,Heilongjiang Academy of Nongken Sciences,Harbin ,China.Effects of cold stress on hypothalamic corticotrophin-releasing and thyrotropin-releasing hormone messenger RNA levels in chickens[J].Journal of Northeast Agricultural University,2007,14(4):327-332. |
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| Authors: | WANG Jintao XU Shiwen |
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| Abstract: | There is increasing evidence that stress can activate the hypothalamic-pituitary-adrenal-axis and hypothalamic-pituitarythyroid-axis, and further affect the synthesis and secretion of corticotrophin-releasing hormone (CRH) and thyrotropin-releasing hormone (TRH). To evaluate the effect of cold stress on the hypothalamic CRH and TRH messenger RNA (mRNA) levels in Yisha chickens, male Yisha chickens were subjected to acute (1, 6, 12 h) and chronic (5, 10, 20 d) cold stress (12±1)℃. Hypothalami were collected for assessment of mRNA levels by semi-quantitative RT-PCR. Acute stress resulted in a significant decrease of CRH mRNA levels at 6 and 12 h, and a significant increase of TRH mRNA levels at every stress time point. Chronic cold stress resulted in a significant increase of CRH mRNA levels and a significant decrease of TRH mRNA levels compared with the control group at every stress time point. The results suggest that the two genes differently respond to cold stress at the mRNA levels. And the different degrees of cold stress will produce different effects on the identical gene. |
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| Keywords: | chicken cold stress semi-quantitative RT-PCR hypothalamus CRH mRNA TRH mRNA |
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