| 鸡新城疫云南分离株HN基因序列测定和分析 |
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| 引用本文： | 赵文华,宋建领,朱建波,肖雷,王金萍,李志华,张念祖.鸡新城疫云南分离株HN基因序列测定和分析[J].黑龙江畜牧兽医,2004(4):13-15. |
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| 作者姓名： | 赵文华 宋建领 朱建波 肖雷 王金萍 李志华 张念祖 |
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| 作者单位： | 云南省热带亚热带动物病毒病重点实验室 云南昆明650224
(赵文华，宋建领，朱建波，肖雷，王金萍，李志华)，云南省热带亚热带动物病毒病重点实验室 云南昆明650224(张念祖) |
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| 摘 要： | 用RT—PCR法对分离自云南省的2株鸡新城疫病毒(编号YN—C1、YN—C2)进行HN基因的扩增和克隆．并对其进行核苷酸序列测定和分析。结果表明：2毒株HN基因开放性阅读框架(ORF)均为l716nt；二者之间的核苷酸同源性为95．3％，氨基酸同源性为95．8％；YN—C1毒株与参考毒株GD／1／98／Go核苷酸、氨基酸同源性均最高，分别为97．9％和97．6％；YN—C2毒株与参考毒株JS／5／01／Go核苷酸同源性最高为98．5％，而与参考毒株JS／3／98／Go氨基酸同源性最高为98．1%。
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| 关 键 词： | 鸡新城疫 RT-PCR 核苷酸同源性 氨基酸同源性 HN基因序列 病毒 毒株 |
| 文章编号： | 1004-7034(2004)04-0013-03 |
| 修稿时间： | 2003年7月3日 |
Sequencing and analysis on the HN gene of newcastle disease virus isolated from chicken in Yunnan |
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| ZHAO Wen-hua,SONG Jian-ling,ZHU Jian-bo,WTBXet al.Sequencing and analysis on the HN gene of newcastle disease virus isolated from chicken in Yunnan[J].Heilongjiang Animal Science And veterinary Medicine,2004(4):13-15. |
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| Authors: | ZHAO Wen-hua SONG Jian-ling ZHU Jian-bo [WTBX] |
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| Institution: | ZHAO Wen-hua,SONG Jian-ling,ZHU Jian-bo,[WT5BX]et al |
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| Abstract: | Virions RNA were abstracted from NDV strain YN-C1 and YN-C2 (isolated from chicken in Yunnan province) and used as templates.The fragment containing hemagglutinin-neuriminidase (HN) gene of the isolates were amplified by RT-PCR method and cloning sequenced.The results showed that:strain YN-C1 and YN-C2 all have 1 716 nt opening reading fragment (ORF) length;the two studied strains have 95.3% nucleotide and 95.8% amino acid homology;strain YN-C1 displays the highest nucleotide (97.9%) and amino acid (97.6%) homology with reference strain GD/1/98/Go;strain YN-C2 demonstrates highest nucleotide homology(98.5%) with strain JS/5/01/Go and highest amino acid homology(98.1%) with strain JS/3/98/Go. |
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| Keywords: | RT-PCR NDV nucleotide homology amino acid homology |
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