|
|||||
|
|
| H1N1亚型猪流感病毒血凝素基因的克隆与表达 | |
| 引用本文： | 马祥,顾万军,刘镇明,黄良宗,彭启明.H1N1亚型猪流感病毒血凝素基因的克隆与表达[J].动物医学进展,2006,27(8):73-76. |
| 作者姓名： | 马祥 顾万军 刘镇明 黄良宗 彭启明 |
| 作者单位： | 1. 华南农业大学兽医学院,广东广州,510642;佛山科学技术学院,广东佛山,528231 2. 佛山科学技术学院,广东佛山,528231 3. 华南农业大学兽医学院,广东广州,510642 |
| 摘 要： | 应用RT-PCR方法扩增了1株H1N1亚型猪流感病毒的血凝素基因,并克隆到pMD 18-T Simple载体中,经PCR、酶切和测序验证克隆正确后,亚克隆到真核表达载体pcDNA3.1( )中,构建了重组质粒pcDNA-HA。在脂质体作用下重组质粒pcDNA-HA转染Vero细胞。间接免疫荧光试验结果表明,血凝素基因在Vero细胞中成功进行了瞬时表达,这为猪流感病毒DNA疫苗的进一步研究奠定了基础。 |
| 关 键 词： | 猪流感病毒 血凝素基因 克隆 表达 Vero细胞 |
| 文章编号： | 1007-5038（2006）08-0073-04 |
| 收稿时间： | 2006-03-16 |
| 修稿时间： | 2006年3月16日 |
Cloning and Expressiong of Subtype H1N1 SIV HA Gene in Vero Cells |
|
| MA Xiang,GU Wang-jun,LIU Zhen-ming,HUANG Liang-zong,PENG Qi-ming.Cloning and Expressiong of Subtype H1N1 SIV HA Gene in Vero Cells[J].Progress In Veterinary Medicine,2006,27(8):73-76. | |
| Authors: | MA Xiang GU Wang-jun LIU Zhen-ming HUANG Liang-zong PENG Qi-ming |
| Institution: | 1. College of Veterinary Medicine,South China Agriculture University , Guangzhou,Guang dong , 510642 , China 2. Foshan College of ,Science and Technology,Foshan,Guangdong,528231,China |
| Abstract: | The HA gene of subtype H1N1 SIV was amplified by RT-PCR assay and sequently subcloned into vector pcDNA3.1( ) to construct expression plasmid pcDNA-HA.Mediated with Lipofectamine TM 2000,pcDNA-HA was introduced into Vero cells.And the indirect immuno fluorescent assay analysis showed that HA gene was been expressed transiently in Vero cells. |
| Keywords: | SIV HA gene clone expression Vero cells |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |