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广东部分地区H1和H3亚型猪流感抗体监测和病毒分离
引用本文:黄良宗,马春全,王淑敏,顾万军.广东部分地区H1和H3亚型猪流感抗体监测和病毒分离[J].畜牧与兽医,2011,43(2).
作者姓名:黄良宗  马春全  王淑敏  顾万军
作者单位:佛山科学技术学院,广东省高校预防兽医学重点实验室,广东,佛山,528231
基金项目:广东现代农业产业技术体系建设专项资金项目,广东省自然科学基金
摘    要:从广东省17个未免疫猪流感病毒(SIV)疫苗的规模化猪场和3个肉联厂的待屠宰猪群中采集2 155份血清样品,采用ELISA方法进行猪流感的血清学调查。结果发现,种猪和肉猪H1亚型猪流感抗体阳性率分别为39.6%(329/830)和37.6%(498/1325),H3亚型抗体阳性率分别为10.8%(90/830)和4.6%(61/1325),H1+H3阳性率分别为5.2%(43/830)和1.8%(25/1325)。从281份猪鼻拭样品和肺样品中分离并鉴定出5株猪流感病毒,其中H1N1亚型3株,H3N2亚型和H1N2亚型各1株。

关 键 词:猪流感  抗体  病毒  鉴定

Serological investigation and viral isolation of H1 and H3 subtype swine influenza in Guangdong
HUANG Liang-zhong,MA Chun-quan,WANG Shu-min,GU Wan-jun.Serological investigation and viral isolation of H1 and H3 subtype swine influenza in Guangdong[J].Animal Husbandry & Veterinary Medicine,2011,43(2).
Authors:HUANG Liang-zhong  MA Chun-quan  WANG Shu-min  GU Wan-jun
Institution:HUANG Liang-zhong,MA Chun-quan,WANG Shu-min,GU Wan-jun(Department of Animal Medicine,Foshan Science and Technology University,Foshan 528231,China)
Abstract:A total of 2155 blood samples were collected from 17 pig farms and the pigs to be slaughtered in Guangdong and the antibody levels of swine influenza virus(SIV) H1 and H3 subtypes were determined by ELISA.The results showed that the positive rates of H1 subtypes antibody of breeding pigs and commercial pigs were 39.6%(329/830) and 37.6%(498/1325),respectively;the positive rate of H3 subtypes antibody were 10.8%(90/830) and 4.6%(61/1325),and the positive rate of H1+H3 subtypes antibody were 5.2%(43/830) and ...
Keywords:swine influenza  antibody  virus  identification  
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