IGF-1在不同鸡与鹌鹑杂交禽胚胎肌肉发育过程中的表达规律与差异

【目的】研究IGF-1基因在蛋用型种鸡(♂)×鹌鹑(♀)和肉用型种鸡(♂)×鹌鹑(♀)两种不同杂交禽胚胎肌肉发育过程中的表达规律,比较该基因在两种不同杂交禽中的差异表达,探讨IGF-1基因在肌肉发育网络调控中的作用。【方法】通过人工受精的方法,蛋用型种鸡(♂)×鹌鹑(♀)获得蛋杂交禽蛋、肉用型种鸡(♂)×鹌鹑(♀)获得肉杂交禽蛋,与鸡蛋、鹌鹑蛋按照鸡标准孵化条件同批人孵,采集第7～17 d活胚胸肌组织,应用实时荧光定量PCR技术检测IGF-1基因在四个物种中每个时间点mRNA的相对表达量。【结果】IGF-1基因在胚胎肌肉发育的第7～17 d均有表达,在第10 d达到第一次峰值,鸡和鹌鹑在第16 d达到第二次峰值,蛋杂交禽和肉杂交禽在第15 d达到峰值。【结论】IGF-1可能在胚胎肌肉的生长发育过程中起调控作用;IGF-1 mRNA表达规律与成肌细胞增殖和肌管融合规律一致,可能参与调控成肌细胞的增殖和肌管的融合。

Developmental Expressional Profiles of IGF-1 during Embryonic Myogenesis in Two Strains of Hybrid of Chicken - Quail

Objective]The purpose of this study was to analyze IGF - 1 mRNA expression profiles and differences during embryonic myogenesis in two strains of hybrid of chicken - quail,and evaluate the potential relationships between IGF - 1 expression and myogenesis.Method]Two strains of cross -bred eggs of quail ($ ) were acquired by artificial insemination by chicken(6 ) - semen,with the eggs of chicken,quail and hybrid in one batch hatched according to the standard condition of chicken,and then the breast muscle tissues from day 7 to day 17 were collected.The expression of IGF - 1 mRNA in different species every day was revealed by real - time quantitative PCR.Result]The expression of IGF - 1 mRNA was detected from day 7 to day 17.The first peak time of IGF - 1 expression was noticed at day 10 in four species,the second peak time was noticed at day 16 in Chicken and Quail,and at day 15 in Meat - Hybrid and Egg - Hybrid. Conclusion]IGF - 1 maybe plays a key role in the process of myogenesis.The expression profile of IGF - 1 was in accord with the profile of myoblasts proliferation and muscle tube fusion,and it maybe plays a key role in the process of myoblasts proliferation and muscle tube fusion.