产海洋细菌MP-2酯酶菌株的鉴定及酯酶理化性质的研究 |
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作者姓名: | 平芮巾 孙谧 刘均忠 王跃军 郝建华 张胜军 |
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作者单位: | 1. 中国水产科学研究院黄海水产研究所海洋酶与酶工程实验室,青岛,266071;大连水产学院,116023 2. 中国水产科学研究院黄海水产研究所海洋酶与酶工程实验室,青岛,266071 3. 青岛市环境保护监测站,266003 |
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基金项目: | 国家自然科学基金,国际科技合作重点计划项目 |
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摘 要: | 从渤海海泥样品中分离获得1株新型酯酶菌株,经鉴定为地衣芽孢杆菌Bacillus licheniformis。所得的MP-2酯酶的最适作用温度范围50~70℃,在60℃表现出了最高活性,属于耐热酶;最适作用pH为10,属于碱性酶,其pH值作用范围比较窄;具有良好的热稳定性;金属离子Co2+,Li+对酶具有激活作用,Ca2+对酯酶的活力没有显著影响,化学试剂SDS、EDTA及Tween-20对酯酶的抑制效果显著,对常见有机溶剂具有良好的耐受力;该酯酶对碳链长短不同的底物表现出不同的酶活。
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关 键 词: | 海洋地衣芽孢杆菌 酯酶 鉴定 性质 |
收稿时间: | 2007/5/22 0:00:00 |
修稿时间: | 2007/7/23 0:00:00 |
Identification of MP-2 esterase producing marine Bacillus and study on properties of the esterase |
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Authors: | PING Rui-jin SUN Mi LIU Jun-zhong WANG Yue-jun HAO Jian-hua ZHANG Sheng-jun |
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Institution: | 1 Laboratory of Marine Enzyme and Enzyme Engineering;Yellow Sea Fisheries Research Institute;Chinese Academy of Fishery Sciences;Qingdao 266071;2 Dalian Fisheries University;116023;3 Environmental Monitoring Station of Qingdao;266003 |
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Abstract: | A novel marine esterase-producing bacteria isolated from the Bohai Sea sediment was identified as Bacillus licheniformis. The MP-2 esterase was primarily purified and characterized. The optimal range of temperature of the esterase was 50-70 ℃, and the maximum activity was achieved at 60 ℃ and pH10.0. The esterase was alkaline and its optimum range of pH was narrow. The thermal stability of the esterase was good. Co^2+,Li+ ions were found to activate the esterase, but Ca^2+ had no significant effects on the activity of the esterase. While SDS, EDTA and Tween-20 had significantly suppressive effects on the esterase. It showed strong resistance to ordinary organic solvents. The enzyme exhibited different esterase activities towards substrates with different length of carbon chains. |
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Keywords: | Marine Bacillus licheniformis Esterase Identification Characteristics |
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