| 滇西北地区5种羊肚菌遗传多样性的ISSR分析 |
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| 引用本文： | 刘文丛,张建博,桂明英,郭相,马明,马绍宾.滇西北地区5种羊肚菌遗传多样性的ISSR分析[J].中国食用菌,2011,30(4):38-42. |
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| 作者姓名： | 刘文丛 张建博 桂明英 郭相 马明 马绍宾 |
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| 作者单位： | 1. 云南大学生命科学学院,云南昆明,650091
2. 中华全国供销总社昆明食用菌研究所,云南昆明,650223 |
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| 基金项目： | “十一五”国家支撑重点项目课题 |
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| 摘 要： | 以滇西北地区11个羊肚茵居群56个羊肚菌样品为研究材料，应用ISSR分子标记方法，进行了遗传多样性与亲缘关系分析。结果表明，11个多态性ISSR引物对全部试验样品进行PCR扩增，共获得88条稳定的条带，其中多态性条带70务（占79．55％）。应用软件POPGENE32分析得出11个羊肚菌居群间遗传距离的变化范围在0．1715～0．4853之间，相似系数的变化范围在0．6155～0．8424之间。遗传分化分析表明，61．35％的变异存在于居群间，38．65％的变异存在于居群内。对56个羊肚菌样品进行分子系统聚类分析（UPGMA）将资源分为三大组，对11个羊肚菌居群进行亲缘关系聚类分析将居群分为两大类群，聚类结果与地理距离有明显的相关性。
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| 关 键 词： | 羊肚菌 ISSR 遗传多样性 遗传分化 亲缘关系 |
Genetic Diversity of Five Species Morchella from Northwest Yunnan Province Revealed by ISSR Analysis |
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| LIU Wen-cong,ZHANG Jian-bo,GUI Ming-ying,GUO Xiang,MA Ming,MA Shao-bin.Genetic Diversity of Five Species Morchella from Northwest Yunnan Province Revealed by ISSR Analysis[J].Edible Fungi of China,2011,30(4):38-42. |
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| Authors: | LIU Wen-cong ZHANG Jian-bo GUI Ming-ying GUO Xiang MA Ming MA Shao-bin |
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| Institution: | LIU Wen-cong1,ZHANG Jian-bo1,GUI Ming-ying2,GUO Xiang2,MA Ming2,MA Shao-bin1(1.Life Science College of Yunnan University,Kunming Yunnan 650091,2.Kunming Edible Fungi Institute of All China Federation of Supply and Marketing Cooperative,Kunming Yunnan 650223) |
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| Abstract: | 11 Morchella populations,including 56 Morchella samples were used to detect the genetic diversity and relationship by inter-simple sequence repeats(ISSR) analysis.The results showed that 88 DNA fragments among all 56 Morchella samples were amplified,using 11 reliable ISSR primers,among which 70 DNA bands were polymorphic(PPB=79.55%).The obtained result by using POPGENE 32 software showed that genetic distance among 11 Morchella populations ranged from 0.1715 to 0.4853,and the genetic identity ranged from 0.... |
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| Keywords: | Morchella ISSR Genetic diversity Genetic variation Genetic relationship |
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