水牛分离精子与不同来源卵母细胞体外受精的效果研究

本研究的目的是探讨水牛分离精子与不同来源(活体采卵或屠宰场卵巢采卵)卵母细胞体外受精的效果。活体采卵是选用20头空怀河流型母水牛(其中摩拉母牛12头,尼里-拉菲母水牛8头)每间隔3 d采卵1次,连续采卵5~6周,活体采集卵母细胞;屠宰场卵巢采卵是收集屠宰场水牛卵巢,用10 mL注射器连接18 G针头吸取水牛卵巢上可视的卵泡来收集卵母细胞。将收集的AB级水牛卵母细胞在相同的条件下进行体外成熟、然后用分离或未分离精子进行体外受精以及体外培养至囊胚。结果发现:活体采卵组和屠宰场收集的水牛卵母细胞组用分离精子受精分裂率和囊胚率没有差异(P>0.05);分离精子和未分离精子的体外受精分裂率和囊胚率也没有差异(P>0.05)。由此说明,水牛分离精子可以用于体外生产性控胚胎。

Efficiency of embryo production in vitro by sexed sperms and oocytes derived from OPU and abattoir ovaries

The objective of the current study was to explore the efficiency of embryo production by sexed sperms and oocytes derived from OPU and abattoir ovaries.Twenty cycling river-type buffaloes(12 Murrah buffaloes,8 Nili-ravi buffaloes) were in the OPU seesions.Retrieval of oocytes from live buffaloes was consecutively carried out once every four days within six weeks and oocytes graded A or B was recovered.Ovaries used for aspiration were derived from buffaloes in abattoir.Oocytes were aspirated from follicles by a syringe equipped with 18G needle and oocytes graded A or B was recovered.Oocytes derived from OPU and abattoir ovaries maturated under the same conditions.Embryo development was evaluated following in vitro fertilized with sexed buffalo sperms.The results revealed that the cleavage and blastocyst development of oocytes derived from OPU and abattoir ovary was not statistically different.And IVF of OPU oocytes with sexed or unsexed sperm was also similar in the cleavage rate and blastocyst development rate,52.80% versus 56.26%(P>0.05) and 20.93% versus 21.81%(P>0.05),respectively.Our results indicated the feasibility of embryo production in vitro using sexed sperm in buffalo species.