| 牛老化卵体外受精后的胚胎发育速度与细胞遗传学研究 |
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| 引用本文： | 朱淑文,李培昌,艾晓杰,华修国,吉泽绿.牛老化卵体外受精后的胚胎发育速度与细胞遗传学研究[J].西南农业大学学报,2002,24(6):498-501. |
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| 作者姓名： | 朱淑文 李培昌 艾晓杰 华修国 吉泽绿 |
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| 作者单位： | [1]上海交通大学农业与生物学院，上海201101 [2]日本国宇都宫大学农学部，日本321－8505 |
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| 基金项目： | 日本国历木县奶牛协会资助项目 (TRN - 1 997- 0 6) |
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| 摘 要： | 体外成熟培养和牛老化卵受精后发育到囊胚到期速度减慢。囊胚期的发育率分别为22h培养组的35．3％，34h组23．6％，46h组15．4％，胚胎的发育速度随培养时间的延长而明显减慢（P＜0．05）。46h组的囊胚期胚胎的平均卵裂球数明显少于22h组（P＜0．05），老化组的染色体平均分裂像，分裂指数均明显低于对照组（P＜0．05）。染色体异常发生率46h组高于22h组。
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| 关 键 词： | 牛老化卵 体外受精 胚胎发育速度 细胞遗传学 囊胚期 卵裂球数 中期分裂像 分裂指数 |
| 文章编号： | 1000-2642(2002)06-0498-04 |
| 修稿时间： | 2002年8月12日 |
THE SPEED OF EMBRYO DEVELOPMENT OF IN VITRO FERTILIZED AGED BOVINE OOCYTES AND THEIR CYTOGENETICAL STUDIES |
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| ZHU Shu-wen ,LI Pei-chang ,AI Xiao-jie ,HUA Xiu-guo ,Yoshizawa Midori.THE SPEED OF EMBRYO DEVELOPMENT OF IN VITRO FERTILIZED AGED BOVINE OOCYTES AND THEIR CYTOGENETICAL STUDIES[J].Journal of Southwest Agricultural University,2002,24(6):498-501. |
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| Authors: | ZHU Shu-wen LI Pei-chang AI Xiao-jie HUA Xiu-guo Yoshizawa Midori |
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| Institution: | ZHU Shu-wen 1,LI Pei-chang 2,AI Xiao-jie 1,HUA Xiu-guo 1,Yoshizawa Midori 2 |
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| Abstract: | The speed of development of in vitro fertilized bovine oocytes to the blastocyst stage markedly decreased after ageing in vitro maturation. The percentage of successful development to blastocyst was 35.4%, 23.6% and 15.4%, respectively, in the 22 h, 34 h and 46 h groups. The speed of development declined with the duration of maturation in vitro (P <0.05). The number of blastomeres of the 46 h group was significantly smaller than that of the 22 h group ( P <0.05). The metaphases and mitotic indexes in the aged groups were significantly lower than those in the control group ( P <0.05). The incidence of chromosomal aberration was significantly higher in the 46 h group than that in the 22 h group. |
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| Keywords: | aged bovine oocyte blastocyst stage blastomere number metaphase mitotic index |
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