何鲁牵牛的组培快繁研究

目的]建立何鲁牵牛的组培快繁体系,为其规模化生产提供理论依据。方法]以何鲁牵牛茎段为外植体,研究何鲁牵牛不定芽增殖和生根的最适培养基。结果]何鲁牵牛在WPM+0.3 mg/L 6-BA+0.01 mg/L NAA+0.1 g/L活性炭的启动培养基上成功诱导腋芽抽出,将腋芽转接到WPM+0.3 mg/L 6-BA+0.1 g/L活性炭培养基上进行不定芽的增殖,增殖率达4.30;在WPM+0.1 mg/L IBA+0.1 mg/L NAA+0.1 g/L活性炭培养基上生根率较高,可达90%。结论]该研究建立了何鲁牵牛的组培快繁体系,有利于何鲁牵牛种质资源保护和工厂化生产。

Study on Tissue Culture and Rapid Propagation of Ipomoea holubii

Objective] To establish tissue rapid propagation system of Ipomoea holubii,and to provide theoretical foundation for its large-scale production.Method] Taking the stem of Ipomoea holubii as explants,we researched the optimal culture medium for adventitious buds prolifera-tion and rooting of I.holubii.Result] Axillary buds were induced on WPM+0.3 mg/L 6-BA +0.01 mg/L NAA +0.1 g/L active carbon (AC).The shoots of I.holubii were cultured on WPM +0.3 mg/L 6-BA +1 g/L AC for multiplication,and the multiplication ratio was 4.30. WPM +0.1 mg/L IBA+0.1 mg/L NAA+0.1 g/L AC was the optimal medium for rooting,and the rooting rate was 90%.Conclusion] This re-search establishes the tissue culture and rapid propagation system of I.holubii,which is helpful to the germplasm protection and industrial produc-tion of I.holubii.