温州乐清湾三个泥蚶群体遗传多样性的初步研究

采用形态判别分析和RAPD分析相结合的方法,对温州乐清湾地区三个泥蚶群体:乐清湾泥蚶,福建泥蚶,和韩国泥蚶三个群体的遗传多样性进行了初步的分析。结果表明:(1)在分子水平上,乐清湾泥蚶群体与韩国群体的亲源关系最为接近,而与地理位置更为接近的福建群体亲缘关系较远。(2)根据形态判别分析,乐清湾泥蚶群体与福建泥蚶群体亲缘最近,而与韩国较远。(3)韩国泥蚶群体的遗传多样性最高,乐清湾泥蚶群体次之,福建泥蚶群体最低。三者的平均遗传杂合度和群体内的遗传距离分别为0.275 4和0.304 8,0.257 5和0.240 1,以及0.247 3和0.211 5,但总体来说,三者的遗传多样性都保持比较高的水平。(4)随机引物S.418,S.359和S.431扩增的结果有乐清湾泥蚶群体特有的条带,其可以作为区分乐清湾泥蚶与其它两种泥蚶的分子标记。

Primary research on the genetic diversity of three populations of Tegillarca granosa in Yueqing Bay

Here we used morphological discrimination analysis and RAPD analysis to study the phylogenetic relationship and genetic diversity of three populations of Tegillarca granosa in Yueqing Bay Wenzhou area.The results showed as follows: (1) The phylogenetic relationship of Yueqing population and Korea population is closer than that of Yueqing population and Fujian population on molecular level, although the geography relationship of Yueqing population and Fujian population is much closer.A possible explanation is that the gene pool of Yueqing population was contaminated by Korea population.(2) However,the phylogenetic relationships of three populations,which are based on morphology data,consisted with the geography relationships.It shows that the degree of contamination of Korea population is not enough to change evidently the shell outline of Yueqing population.(3) From highest to lowest,the genetic diversity of three populations is Korea,Yueqing,and Fujian.Their average heterozygosity is(0.275 4),(0.257 5) and (0.247 3),and the average genetic distance of each population is 0.304 8,0.240 1 and 0.211 5,respectively.These also show that the genetic diversities of the three populations maintained high level.(4) There 4 specific bands appeared in Yueqing population using random primers S.418,(S.359) and S.431 PCR amplification,and they could be used as molecular markers of Yueqing population.