内生真菌gyzy-20生产高三尖杉酯碱的成分分析及条件优化 |
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引用本文: | 韩洁,赵杰宏.内生真菌gyzy-20生产高三尖杉酯碱的成分分析及条件优化[J].安徽农业科学,2010,38(34):19291-19293. |
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作者姓名: | 韩洁 赵杰宏 |
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作者单位: | 贵阳中医学院基础部微生物教研室,贵州贵阳,550002;贵州省烟草科学研究所,贵州贵阳,550081 |
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基金项目: | 贵州省卫生厅优秀医学青年科技人才专项科研基金项目,贵州省中医药管理局中医药、民族医药科学技术研究专项课题项目,贵阳中医学院青年科研基金项目 |
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摘 要: | 目的]为充分发掘篦子三尖杉(Cephalotaxus oliveriMast)内生真菌的应用价值。方法]采用HPLC对内生真菌菌株gyzy-20的发酵提取组分进行鉴定,并采用正交试验进一步优化生产工艺。结果]菌株gyzy-20的代谢产物中有高三尖杉酯碱生成,提取时间对高三尖杉酯碱含量的影响较大。优化的生产工艺为:碳源为葡萄糖,发酵10 d后超声5 min,添加2倍体积二氯甲烷萃取3 h,优化后的发酵和提取方法可提高高三尖杉酯碱的含量1.5倍以上,达到0.26μg/ml。结论]该研究可为利用微生物发酵生产HHT提供可能。
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关 键 词: | 内生真菌 高三尖杉酯碱 液相色谱 发酵条件 优化 |
Components Analysis of Homoharringtonine Produced by Endophytic Fungi Strain gyzy-20 and Optimization for Fermentation Condition |
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Institution: | HAN Jie et al(Lab of Microbiology,Guiyang College of Traditional Chinese Medicine,Guiyang,Guizhou 550002) |
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Abstract: | Objective]In order to explore the application value of endophytic fungi isolated from Cephalotaxus oliveri Mast.Method]The fermentation products of strain gyzy-20 were extracted and analyzed by HPLC,and then the producing process was optimized by orthogonal experiment.Result]The metabolites of strain gyzy-20 contained homoharringtonine(HHT),in which the HHT content was affected by extraction time largely.The optimized producing process was as follow:C sources glucose,ultrasonic extraction 5 min after fermenting 10 d,extraction 3 h after adding 2 times volumes of dichloromethane.The HHT content up to 0.26 μg/ml of fermentation broth,increased more than 1.5 times through optimized producing process comparing with that of before.Conclusion]The study can provide probability on HHT production through microbe fermentation. |
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Keywords: | Endophytic fungi Homoharringtonine HPLC Fermentation condition Optimizing |
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