HPLC法测定芦荟中芦荟苷的含量及其色谱图特征分析

目的]建立芦荟中芦荟苷含量的HPLC测定方法,测定几种芦荟中芦荟苷的含量,并分析这几种芦荟色谱图的特征,从而将5种芦荟区分开来。方法]采用超声振荡提取法制备样品溶液,并用HPLC测定芦荟苷的含量,得到芦荟色谱图。结果]芦荟苷的线性回归方程为Y=13.168 4X-2.855 4,R=0.999 8,表明在2.92～46.67μg/ml范围内,芦荟苷浓度与峰面积的线性关系良好,平均回收率为108.0%;5种芦荟苷的含量分别为:木立芦荟614.3μg/g、海虎兰212.5μg/g、库拉索芦荟135.3μg/g、华芦荟72.92μg/g和不夜城芦荟2.622μg/g。库拉索芦荟在3～5 min出现的双峰左边的峰高稍低,但相差不明显,双峰基本达到基线分离,在其后再没有明显的色谱峰。该特征仅与木立芦荟的色谱图相似,与华芦荟、不夜城芦荟和海虎兰色谱图相差较远,较易区分。结论]不同品种芦荟中芦荟苷的含量不同;其中木立芦荟中芦荟苷的含量最高;所确立的检测方法简便,准确,快速。图谱分析可将这5种芦荟区分开来。

Determination of Aloin Content in Aloe with HPLC and Analysis of Aloe Chromatogram

Objective] To establish a HPLC method for determining the content of aloin in 5 species of Aloe,and analyze their chromatograms,so as to distinguish them from each other.Method] The content of aloin in sample solutions of Aloe species prepared using ultrasonic wave method was measured by HPLC.And,the chromatograms of Aloe were obtained.Result] The linear regression equation of aloin was Y=13.168 4X-2.855 4,R=0.999 8,showing good linearity between concentration and peak area in the range of 2.92 to 46.67 μg/ml.The average recovery rate was 108.0%.The content of aloin was 614.3 μg/g for A.arborescens,212.5 μg/g for A.delaetii,135.3 μg/g for A.vera L.,72.92 μg/g for A.vera L.var.chinesis(Haw.) Berg,and 2.622 μg/g for A.nobilis.Double peaks occurred at the retention time of 3 to 5 min in the HPLC chromatogram of A.vera L.,the peak height of which was slightly higher on the left,but this difference was not significant.Its double peaks were basically separated at the baseline.From then on,no obvious chromatographic peaks appeared on the chromatogram.In addition,the chromatogram of A.vera L.was similar to that of A.arborescens and very different from that of other three species.Conclusion] There is a clear difference in aloin level among 5 species of Aloe.Of these,the content of aloin in A.arborescens is the highest.The determination method is stable,accurate and quick.Five species of Aloe are distinguished from each other by means of chromatogram analysis.