β-氨基丁酸诱导烟草抗烟草花叶病毒系统侵染的研究

目的]研究阻氨基丁酸（BABA）对烟草抗烟草花叶病毒（TMV）系统侵染的影响。方法]以烟草普通栽培种为研究对象，用BABA进行处理．处理后所有的烟苗均放入保湿袋中保湿24h。然后用组织化学法鉴定BABA诱导烟草产生的过敏反应，并进行产生H2O2，原位检测以及活性氧和丙二醛测定。（结果]BABA处理后叶片中的，β-1，3-葡聚糖酶活性升高，1MV接种后叶片中该酶活性也进一步升高。10mmol／LBABA喷洒处理能显著诱导氧进发，引起过敏性反应，叶面局部细胞坏死。处理叶片中的过氧阴离子自由基、丙二醛含量增加。10mmol／LBABA有效激发植株的系统抗性，限制TMV的侵染，增强了烟草对TMV的抗性。结论]BABA可增强普通烟草栽培品种抗TMV系统侵染的能力。但其诱抗能力要受其浓度的影响。

Resistance Induced by β-aminobutyric Acid in Tobacco to Tobacco Mosaic Virus

Objective] The study aimed to research the effect of β-Aminobutyric acid(BABA) on the resistance in tobacco to the systemic infection of tobacco mosaic virus(TMV).Method] With the common cultivated species of tobacco as the researched object,it was treated by BABA.All the tobacco seedlings were put into the moisture retention bag to keep moisture for 24 h after treatment.Then the allergic reaction of inducing tobacco generation by BABA with histochemistry method,the generation H2O2 in suit was measured and reactive oxygen species and malondialdehyde were measured.Result] The activity of β-1,3-glucanase in leaves was improved remarkably after treatment by BABA,which was also improved in leaves after TMV inoculation.The sprinkle treatment of 10 mmol/L BABA could obviously induce oxygen burst,cause hypersensitive response,make foliar local cellular necrosis.The content of superoxide anion radicals and malondialdehyde in tobacco leaves was increased after BABA treatment.10 mmol/L BABA could effective induce systemic acquired resistance,prevent tobacco from TMV invading and enhance resistance to TMV in tobacco plants.Conclusion] BABA could improve the ability of the common cultivated species of tobacco on resistance TMV systemic infection,but its induce resistance ability was influenced by its concn..