| 清肺颗粒HPLC指纹图谱建立及(R,S)-告依春含量测定 |
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| 引用本文： | 刘雪松,张艳,苏景,郝敬友,唐伟,刘彦凯,孙洪杰,徐丽,薛沾枚,史同瑞.清肺颗粒HPLC指纹图谱建立及(R,S)-告依春含量测定[J].动物医学进展,2022,43(2):80-84. |
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| 作者姓名： | 刘雪松 张艳 苏景 郝敬友 唐伟 刘彦凯 孙洪杰 徐丽 薛沾枚 史同瑞 |
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| 作者单位： | 黑龙江省农业科学院畜牧兽医分院,黑龙江齐齐哈尔161005,黑龙江省动物疫病控制中心,黑龙江哈尔滨150030,哈尔滨绿达生动物药业有限公司,黑龙江哈尔滨150030 |
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| 基金项目： | 黑龙江省重点研发计划项目 |
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| 摘 要： | 为了建立中兽药清肺颗粒(板蓝根、葶苈子、桔梗等)HPLC指纹图谱,同时测定其中(R,S)-告依春的含量.将该复方药的提取液进行分析,采用Inert SustainC18色谱柱(250 mm×4.6 mm,5 μm),流动相为乙腈-5 mL/L甲酸溶液,梯度洗脱,流速为1 mL/min,柱温30℃,检测波长254 nm,...
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| 关 键 词： | 清肺颗粒 指纹图谱 液相色谱 (R S)-告依春 |
HPLC Fingerprint Study and Determination of Epigoitrin of Qingfei Granules |
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| LIU Xue-song,ZHANG Yan,SU Jing,HAO Jing-you,TANG Wei,LIU Yan-kai,SUN Hong-jie,XU Li,XUE Zhan-mei,SHI Tong-rui.HPLC Fingerprint Study and Determination of Epigoitrin of Qingfei Granules[J].Progress In Veterinary Medicine,2022,43(2):80-84. |
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| Authors: | LIU Xue-song ZHANG Yan SU Jing HAO Jing-you TANG Wei LIU Yan-kai SUN Hong-jie XU Li XUE Zhan-mei SHI Tong-rui |
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| Institution: | (Animal Husbandry and Veterinary Institute,Heilongjiang Academy of Agricultural Science,Qiqihar,Heilongjiang,161005,China;Heilongjiang Provincial Animal Disease Prevention and Control Center,Harbin,Heilongjiang,150030,China;Harbin Lvdasheng Animal Pharmaceutical Co.,Ltd,Harbin,Heilongjiang,150030,China) |
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| Abstract: | In order to establish the HPLC fingerprint of Qingfei granules(Isatidis Radix,Lepidii Semen,Platycodon grandiflorus,etc.)and simultaneously determine the content of epigoitrin,the HPLC fingerprint was established by using InertSustain C18 column(250 mm×4.6 mm,5μm),acetonitrile-0.5%formic acid solution as mobile phase,gradient elution,flow rate of 1 mL/min,column temperature of 30℃,detection wavelength of 254 nm.There were 12 common peaks in the fingerprints of 10 batches of samples,and the similarity was more than 0.9.The linear range of epigoitrin was 0.8μg/mL-24μg/mL.the recovery was 98.63%,RSD was 1.56%.The average content epigoitrin of in 10 batches of granule samples was 0.0161 mg/g.The results showed that the method had good systematicness and stability,and could be used for the quality control of Qingfei granules. |
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| Keywords: | Qingfei granules fingerprint HPLC epigoitrin |
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