高效液相色谱法测定水果罐头中苯甲酸、山梨酸和糖精钠

目的]建立测定水果罐头中苯甲酸、山梨酸和糖精钠的高效液相色谱法。方法]通过回收率、精密度和重现性试验建立了测定水果罐头中苯甲酸、山梨酸和糖精钠的高效液相色谱法。结果]通过紫外光谱扫描,确定230 nm作为测定波长。在0.010~0.200 g/L范围内,苯甲酸、山梨酸和糖精钠的回归方程相关系数均大于0.99,检出限分别为0.0100、.004和0.002 g/L。水果罐头中苯甲酸、山梨酸和糖精钠的添加回收率在94.0%~102.0%,变异系数在0.56%~2.15%。按样品测定方法平行测定5次,苯甲酸、山梨酸和糖精钠的峰面积相对标准偏差(RSD)分别为0.92%、0.79%和0.95%。结论]该法测定水果罐头中的苯甲酸、山梨酸和糖精钠具有良好的准确性和精密度。

Determination of Benzoic Acid, Sorbic Acid and Saccharin Sodium in Fruit Can by HPLC

Objective] The study aimed to establish the HPLC method of determining benzoic acid,sorbic acid and saccharin sodium in fruit can. Method] The HPLC method of determining benzoic acid,sorbic acid and saccharin sodium in fruit can was established through recovery,precision and repeatability experiments.Result] The wavelength of 230 nm was confirmed to be determination wavelength through ultraviolet scan.In the range of 0.010～0.20 g/L,the correlation coefficients of regression equations of benzoic acid,sorbic acid and saccharin sodium were bigger than 0.99 with detection limits of 0.010,0.004 and 0.002 g/L resp.The recoveries of benzoic acid,sorbic acid and saccharin sodium in fruit can were in 94.0%～102% with variation coefficients in 0.56%～2.15%.The RSDs of peak areas of benzoic acid,sorbic acid and saccharin sodium in 5 parallel determinations by sample-determination method were 0.92%,0.79% and 0.95% resp.Conclusion] Determination of benzoic acid,sorbic acid and saccharin sodium in fruit can by this method had good accuracy and precision.