| 兰州百合微繁的研究 |
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| 作者姓名： | 徐凌飞 庞 勇 周连霞 王贵章 王 婧 叶红霞 |
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| 作者单位： | 西北农林科技大学园艺学院 陕西杨凌712100
(徐凌飞，周连霞，王贵章，王婧)，甘肃农业职业技术学院 甘肃兰州730020
(庞勇)，西北农林科技大学园艺学院 陕西杨凌712100(叶红霞) |
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| 摘 要： | 以兰州百合的鳞茎增殖为基础,进行了离体微繁研究。鳞茎增殖的适宜激素组合为0.9mg/LBA 0.1~0.2mg/LNAA。培养基中总离子浓度和氮浓度过高或过低都会影响兰州百合增殖,3种基本培养基N6、MS、和1/2MS以N6增殖效果最好。适当提高糖浓度对鳞茎增殖有促进作用,在各处理中以蔗糖浓度为40g/L增殖倍数高。兰州百合的适宜生根培养基为1/2MS IBA0.1mg/L,生根率达100%,生根苗移栽成活率达90%。
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| 关 键 词： | 兰州百合 鳞茎 离体繁殖 |
Micropropagation of Lilium davidii var. unicolor |
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| Authors: | Xu Lingfei Pang Yong Zhou Lianxi Wang Guizhang Wang Jing Ye Hongxia |
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| Institution: | (1 College of Horticulture, Northwest Sci-Tech University of Agriculture and Forestry,Yangling,shaanxi 712100;
2 Gansu Agriculture Technology College, Lanzhou, Gansu 730020) |
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| Abstract: | An in vitro propagation protocol based on bulb proliferation has been developed for Lilium davidii var. unicolor . BA 0.9 mg/L NAA 0.1~0.2 mg/L was suitable for bulb proliferation .It seemed that high or low total ion and nitrogen concentration affected bulb proliferation. N6 was optimal basal medium for bulb proliferation. Improving sugars concentration enhanced the multiplication efficiency. The multiplication efficiency of 40g/L Sucrose was higher than others. The best rooting response was obtained on 1/2 MS medium supplemented with 0.1mg/L IBA, on this medium; the rooting frequency was 100%. The survival percentage was 90% when rooting plantlets were transplanted in perlite and soil. |
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| Keywords: | Lilium davidii var unicolor Bulb In vitro propagation |
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