扶芳藤遗传多样性RAPD鉴定及类型划分研究

利用RAPD标记技术对56份扶芳藤、3份大叶黄杨和1份胶东卫矛材料进行遗传多样性分析,筛选出35个引物扩增出205条稳定、清晰、可重复的DNA片段,其中32个引物扩增出100条带具有多态性,其多态性频率48.78%;不同引物及不同位点谱带所揭示的扶芳藤遗传差异较大,其遗传多样性指数分布范围为0.0331￣0.8904;根据引物L04和U12扩增的RAPD带型和以遗传距离进行聚类分析结果可明显地区分扶芳藤与大叶黄杨、冬季全部落叶的与冬季不落或部分落叶的扶芳藤类型、从中国收集的扶芳藤资源与从捷克和美国引入的3个品种;同时聚类分析还可区分中国东南沿海地区与其他地区收集扶芳藤资源。

Study on Genetic Diversity Identification by RAPD Makers and Classification in Euonymus fortunei

56 Euonymus fortunei clones, 3 Euonymusclones and 1 Euonymus clone were analyzed with RAPD. Using 35 arbitrary primers, we scored a total of 205 stable, clear and repeatable DNA fragments. Using 32 of these primers we scored 100 polymorphic bands. The average polymorphism rate was 48.78%. Different primer and different location revealed significantly genetic difference. The range of genetic diversity index (GDI) was from 0.0331 to 0.8904.Through the RAPD bands revealed by primer L04 and U12 and the cluster analysis of genetic distance we can obviously differentiate Euonymus fortunei and Euonymus, defoliate, not defoliate and partly defoliate type of Euonymus fortunei in winter, the breed collected from our country and the 3 breeds introduced from Czechic and America . Cluster analysis can also differentiate theEuonymus fortunei resources collected from southeast littoral and other areas of our country.