| 利用微卫星标记鉴定金优207杂种纯度技术研究 |
| |
| 引用本文： | 薛灿辉,朱克永,陈祖武,詹庆才.利用微卫星标记鉴定金优207杂种纯度技术研究[J].湖南农业科学,2005(5):6-8. |
| |
| 作者姓名： | 薛灿辉 朱克永 陈祖武 詹庆才 |
| |
| 作者单位： | 湖南省水稻研究所,湖南,长沙,410125 |
| |
| 基金项目： | 湖南省科技厅资助项目（01NKY1002） |
| |
| 摘 要： | 利用微卫星分子标记,对杂交组合金优207的双亲及F1之间的多态性进行引物筛选和检测技术研究.结果如下：在已筛选的232对引物中,有32对引物显示稳定的多态性,杂种表现为父母本互补的带型.用表现多态性的两对引物分别对人为掺假的金优207进行纯度鉴定,能将掺入组合中的假种子区分开.目前采用的方法,具有准确可靠、多态性高、稳定性好、易于操作等特点.
|
| 关 键 词： | 杂交水稻 微卫星标记 纯度鉴定 |
| 文章编号： | 1006-060X(2005)05-0006-03 |
| 收稿时间： | 2005-05-12 |
| 修稿时间： | 2005年5月12日 |
Studies on Identification and Factuality of Jingyou207 Series Using SSR marker |
| |
| XUE Can-hui,ZHU Ke-yong,CHEN Zu-wu,ZHAN Qing-cai.Studies on Identification and Factuality of Jingyou207 Series Using SSR marker[J].Hunan Agricultural Sciences,2005(5):6-8. |
| |
| Authors: | XUE Can-hui ZHU Ke-yong CHEN Zu-wu ZHAN Qing-cai |
| |
| Institution: | Hunan Academy of Agricultural Science Rice Research Institute, Changsha 410125, PRC |
| |
| Abstract: | 232 pairs of SSR primers distributing in 12 rice chromosomes were used to detect Jingyou207 series. Out of the used primers, 32 were repeatable polymorphic. It could distinguish all restorer lines and sterile lines effectively. The bands of hybrid rice were complement types of their parents, which were adapted to examine purity of hybrid rice seed. The possibility of economical beneficial on identification of hybrid rice purity was discussed. |
| |
| Keywords: | hybrid rice SSR marker purity identification |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
