小麦不同部位DNA提取的比较研究

目的]探讨小麦不同取材部位对DNA提取质量和产率的影响。方法]分别以小麦的种子、根尖和幼嫩叶片为材料，采用改良CTAB法分别提取小麦3个部位的基因组DNA，用0．8％的琼脂糖凝胶电泳检测DNA质量，用紫外分光光度计分别测定3个部位的DNA在A260和A280下的吸光值，根据A260/A280的值检测DNA的浓度和纯度，并以3个部位提取的DNA为模版进行RAPD扩增。结果]采用小麦叶片提取DNA的产率最高，种子次之，根尖最低。3个部位提取的DNA纯度均相近，提取叶片获得的DNA浓度最大，为756μg/ml；其次是种子，为233μg/ml；根尖获得的DNA浓度最低，为90μg/ml。小麦不同部位提取的DNA模板对RAPD扩增没有明显影响，DNA浓度都能达到扩增的要求。结论]小麦3个部位提取的DNA质量均较好，可以进行后续的酶切和PCR扩增等实验。

Comparative Study on Extraction of Genomic DNA from Different Parts of Wheat

Objective] The aim was to discuss the effect of different sampling positions of wheat on the DNA quality and yield.Method] With seeds,root tips and young leaves of wheat as tested materials,the genome DNA from 3 parts of wheat were extracted with modified CTAB method and examined with 0.8% agarose gel electrophoresis.The absorbance value of DNA from 3 parts was measured by ultraviolet spectrophotometer at A260 and A280 and the ratio of A260/A280 was used to determine the DNA purity and the mass concn.DNA ...