| 水貂自咬症SCAR标记的筛选 |
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| 引用本文： | 刘宗岳,杜智恒,杨春山,李秋芳,白秀娟.水貂自咬症SCAR标记的筛选[J].东北林业大学学报,2010,38(2). |
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| 作者姓名： | 刘宗岳 杜智恒 杨春山 李秋芳 白秀娟 |
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| 作者单位： | 东北农业大学,哈尔滨,150030;东北农业大学,哈尔滨,150030;东北农业大学,哈尔滨,150030;东北农业大学,哈尔滨,150030;东北农业大学,哈尔滨,150030 |
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| 摘 要： | 采用随机扩增多态DNA(RAPD)技术对健康和自咬水貂群体进行检测,并在此基础上进行了RAPD向序列特异性扩增(SCAR)标记的转化。从100个RAPD随机引物中筛选出5个重复性好的引物。通过对引物(A1)的扩增能够在两群体中找到差异标记(HA-400)和共有标记(SA-500),对其进行克隆、测序,并根据测序结果设计两对SCAR引物(SHA-400和SSA-500)。SHA-400和SSA-500在健康和自咬群体中均有扩增。其中,SHA-400在两个群体扩增频率分别为82.5%和22.5%,差异极显著(P0.001);SSA-500在两群体的扩增频率为87.5%和97.5%,差异不显著(P0.05)。结果表明:HA-400可初步作为区分健康和患病水貂群体的分子遗传标记。
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| 关 键 词： | 水貂 自咬行为 RAPD SCAR |
Screening of SCAR Marker Linked to Mink Self-biting Disease |
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| Liu Zongyue,Du Zhiheng,Yang Chunshan,Li Qiufang,Bai Xiujuan.Screening of SCAR Marker Linked to Mink Self-biting Disease[J].Journal of Northeast Forestry University,2010,38(2). |
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| Authors: | Liu Zongyue Du Zhiheng Yang Chunshan Li Qiufang Bai Xiujuan |
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| Institution: | College of Animal Science & Technology;Northeast Agricultural University;Harbin 150030;P.R.China |
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| Abstract: | RAPD was used to analyze genetic constitution of the healthy and self-biting mink groups,and was converted into SCAR marker.Five polymorphic primers were screened from 100 random primers by RAPD.Specific and non-specific amplified DNA fragments,HA-400 and SA-500,were found,cloned and sequenced.Based on these sequences,two specific primers of SCAR were designed(SHA-400 and SSA-500).Then PCR amplification was carried out for the healthy and self-biting minks.Results showed that almost all of mink were amplifi... |
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| Keywords: | Mink Self-biting behaviors RAPD SCAR |
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