| RAPD分析中大豆基因组DNA的快速制备 |
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| 引用本文： | 苏颖,王鼐,王江红,赵洪锟,郭中校,董英山.RAPD分析中大豆基因组DNA的快速制备[J].吉林农业科学,2006,31(3):35-37. |
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| 作者姓名： | 苏颖 王鼐 王江红 赵洪锟 郭中校 董英山 |
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| 作者单位： | 吉林省农业科学院,吉林,公主岭,136100 |
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| 摘 要： | 提出一种简便、实用的DNA提取方法,DNA得率可达到80μg/g,所得DNA样品的OD260/OD280值在1.8以上,且不含PCR反应抑制剂,RAPD扩增结果良好,该方法提取的DNA产量和质量均能够满足基因工程操作的要求。
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| 关 键 词： | RAPD 大豆 基因组DNA 提取 |
| 文章编号： | 1003-8701（2006）03-0035-03 |
| 收稿时间： | 2006-04-10 |
| 修稿时间： | 2006年4月10日 |
A Method of Extracting DNA from Soybean Used in RAPD Analysis |
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| SU Ying, WANG Nai, WANG Jiang-hong,et al..A Method of Extracting DNA from Soybean Used in RAPD Analysis[J].Jilin Agricultural Sciences,2006,31(3):35-37. |
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| Authors: | SU Ying WANG Nai WANG Jiang-hong |
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| Institution: | Academy of Agricultural Sciences of Jilin Provinces, Gongzhuling, 136100, China |
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| Abstract: | A simple and practical method of DNA extraction was described in this paper.The effective rate of DNA reached 80μg/g.The value of OD260/OD280 of DNA simple was more than 1.8,and there was not inhibitor of polymerase chain reaction.The results of RAPD were all right.The quantity and quality of DNA simple extracted by this method could satisfy the requirement of genetic engineering operations. |
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| Keywords: | RAPD Soybean Genomic DNA |
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