REV和ALV-J感染对肉用型鸡细胞免疫反应的影响

以网状内皮增生症病毒（REV）和禽白血病病毒J亚群（ALV-J）单一感染和共感染1日龄商品代AA肉鸡后不同时间,采用3H-TdR掺入法测定血液和脾脏的淋巴细胞对ConA的增殖反应能力。结果表明,血液淋巴细胞对ConA增殖反应能力在REV和ALV-J共感染后7 d均下降,REV单一感染组在感染后17、37 d均极显著低于对照组（P〈0.01）,ALV-J单一感染组也呈现下降趋势。在脾淋巴细胞反应中,REV感染组在感染后37 d极显著降低（P〈0.01）,ALV-J组显著降低（P〈0.05）。REV和ALV-J共感染抑制淋巴细胞对ConA增殖反应较单一感染强,效应期也较长,在感染后37 d,共感染对血液和脾淋巴细胞反应的抑制作用均大于REV和ALV-J的单一感染（P〈0.05）。在感染后273、7 d检测NDV抗体,单一感染组降低显著（P〈0.05）,而共感染组下降极显著（P〈0.01）,且显著低于单一感染组（P〈0.05）。本研究表明REV、ALV-J感染不仅能抑制体液免疫反应,也能抑制细胞免疫反应,且共感染比单一感染的抑制作用更强。

Influence of REV and ALV-J infection on cellular immunity responses in commercial broiler chickens

To explore the influence of reticuloendotheliosis virus(REV) and subgroup J avian leucosis virus(ALV-J) infection on chicken cellular immunity,the mitogenic response to ConA of blood and spleen lymphocytes tested and analyzed with 3H-thymidine(3H-TdR) incorporation assay at different intervals after infection or co-infection of single or both viruses.Results indicated that mitogenic responses to ConA of blood lymphocytes decreased significantly in REV-infected birds than in controls(P<0.01) at days 17 and 37 after infection.The responses of lymphocytes from birds infected with ALV-J decreased but not significantly.However,mitogenic responses of spleen lymphocytes decreased significantly in birds infected with REV(P<0.01) or ALV-J(P<0.05) alone when compared to the controls.Co-infection of REV and ALV-J inhibited the general cellular immunity more severely than in single infection.ConA-stimulated response of both blood and spleen lymphocytes from birds co-infected with REV and ALV-J were lower than that from birds infected with REV or ALV-J alone significantly(P<0.05) at 37 days after infection.Single infection with REV or ALV-J decreased significantly(P<0.05) HI-antibody titers to NDV after vaccination with inactivated vaccine.HI titers in birds co-infected with REV and ALV-J were also lower than REV or ALV-J single infection(P<0.05).The study demonstrated that the infection of REV and ALV-J enable to suppress ont only the humoral immunity,but also the cellular immunity,and co-infection with both viruses may cause immunosuppression more severely than single infection of REV or ALV-J.